慢性根尖周炎对大鼠血浆炎症因子及主动脉的影响  

Effect of Chronic Apical Periodontitis on Plasma Cytokine and Aorta Intima in Rats

作　　者：马典福 陈帅[2,3] 肖素丽 李萍萍 黄晓晶 MA Dianfu;CHEN Shuai;XIAO Suli;LI Pingping;HUANG Xiaojing(Department of Stomatology,Xiamen Children's Hospital,Xiamen 361006,China;School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China;Key Laboratory of Stomatology,Fujian Province University,Fuzhou 350002,China;Xiamen Stomatological Hospital,Xiamen 361000,China)

机构地区：[1]厦门市儿童医院口腔科,福建厦门361006 [2]福建医科大学附属口腔医院,福建福州350002 [3]福建省高校口腔医学重点实验室,福建福州350002 [4]厦门市口腔医院,福建厦门361000

出　　处：《口腔医学研究》2019年第9期872-875,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81500845、81271131);福建省自然科学基金(编号:2017J01521);福建省医学创新课题(编号:No.2018-CX-37);福建省卫生计生委青年科研课题(编号:No.2015-1-64)

摘　　要：目的:研究大鼠根尖周炎慢性感染12周对血浆炎症因子及主动脉的影响。方法:32只雄性SD大鼠分别经普通饮食(Normal Diet,ND组)和高脂饮食(High Fat Diet,HFD组)喂养9周后,根据磨牙开髓数目ND组再分为N0(假手术组)、N6(6颗开髓),HFD组再分为H0、H6组。第24周,各组大鼠心脏采血测量血脂(TC、TG、HDL-C、LDL-C)、炎症因子(CRP、IL-2、IL-6、IL-10),主动脉样本HE和苏丹Ⅳ染色。结果:CRP、IL-2、IL-6、IL-10各组间差异无统计学意义(P>0.05)。HE染色示N0组主动脉内膜光滑,无明显炎症细胞黏附;N6、H0、H6组见少量炎症细胞位于主动脉肌层和外膜。苏丹Ⅳ染色示各组主动脉壁未见明显红染条纹、斑块等。结论:大鼠根尖周炎慢性感染12周时,即使合并高脂血症,血浆炎症因子未见明显异常,主动脉少量炎症细胞浸润。Objective:To investigate the effect of chronic apical periodontitis (CAP) for 12 weeks on rat plasma cytokines response and lesion of aorta.Methods:32 male SD rats were randomly divided into high fat diet group and normal diet group.After 9 weeks,according to the teeth number of opening pulp cavity,the rats were divided into four groups ( n =8) to build CAP model,namely N0,N6,H0 and H6.After 24 weeks,plasma concentrations of lipid (TC,TG,HDL-C,LDL-C) and inflammation cytokine (CRP,IL-2,IL-6,IL-10) were detected.Histological examinations of aorta were performed by HE staining and sudan Ⅳ staining.Results:There were no significant difference in plasma CRP,IL-2,IL-6 and IL-10 among four groups ( P >0.05).Aorta intima was smooth without obvious inflammation cell adhesion in N0 group.A small amount of inflammatory cells were found in muscularis and adventitia among N6,H0 and H6 groups.Conclusion:CAP for 12 weeks on rats did not raise plasma concentration of cytokine,neither did it when combined with hyperlipidemia.CAP infection caused slight inflammatory cells infiltration in rat aorta.

关 键 词：慢性根尖周炎 炎症因子 主动脉 

分 类 号：R781.341[医药卫生—口腔医学]