细菌Chryseobaterium sp.溶藻方式及其溶藻胶囊制备条件研究  

THE WAY OF DISSOLVING ALGAE by CHRYSEOBATERIUM SP. AND PREPARATION OF ALGAE-DISSOLVING CAPSULES

作　　者：王金霞[1] 陈玉成[2] 詹玲玲 罗乐[1] WANG Jin-xia;CHEN Yu-cheng;ZHAN Ling-ling;LUO Le(Chongqing Vocational Institute of Engineering,Chongqing 402260,China;Resource and EnvironmentSciences,Southwest University,Chongqing 400715,China)

机构地区：[1]重庆工程职业技术学院,重庆402260 [2]西南大学资源环境学院,重庆400715

出　　处：《环境工程》2019年第9期91-96,193,共7页Environmental Engineering

基　　金：国家科技支撑计划(2015BAL04B01);重庆市教育委员会科学技术研究计划青年项目(KJQN201803406)

摘　　要：为探究细菌 Chryseobaterium sp.的溶藻能力及方式,通过扫描电镜观察细菌 Chryseobaterium sp.对藻 Anabaena flos-aquae 的作用效果,进一步通过分离菌体和菌上清液的方法探讨其溶藻的作用机制。结果表明:细菌 Chryseobaterium sp.具有很强的溶藻能力,其主要通过分泌溶藻活性物质进行间接溶藻。胶囊制备的优化条件为:SA、CaCl 2、EC和柠檬酸质量分数分别为3%、3%、3%和2%,CaCO 3与SA的质量分数比为4/5,交联时间为30 min,溶藻物质质量分数为16%,该条件下所制胶囊缓慢释放溶藻物质,具有长效的溶藻能力。揭示了细菌 Chryseobaterium sp.主要通过分泌溶藻活性物质进行间接溶藻,所制胶囊具有较强的溶藻能力,为细菌 Chryseobaterium sp.溶藻机理和生物控藻技术的研究提供了理论依据。To explore the capability and mode of bacterial Chryseobaterium sp.dissolving alga Anabaena flos-aquae , the dissolving course was observed by the scanning electron microscopy.The direct (bacteria) and indirect (bacteria suspension) modes were also observed.The results showed that bacterial Chryseobaterium sp.was with strong ability in dissolving algae, and mainly through the secretion of algae-lysing substances indirectly.The optimum conditions for making algae-dissolving capsules were as follows: SA mass ratio of 3%, CaCl 2 of 3%, EC of 3%, citric acid of 2%, the ratio of CaCO 3 to SA of 4/5, and the crosslinking time of 30 min.The capsules had long-term capacity in dissolving algae by slowly release the algae-lysing substances.The results revealed that bacteria Chryseobaterium sp.possessed a strong ability of activate substances indirectly through the secretion of algae-lysing, which provided a theoretical basis for studying the mechanisms of Chryseobaterium sp.dissolve algae, and also a new thinking in controlling algae growth by biotechnology.

关 键 词：溶藻细菌 Chryseobateriumsp. Anabaenaflos-aquae 细菌上清液 溶藻胶囊 

分 类 号：X172[环境科学与工程—环境科学] X52