常见电子介体对大肠杆菌微生物燃料电池产电性能的影响  

Effects of different electron mediators on the performance of Escherichia coli BL21(DE3) microbial fuel cell

作　　者：刘海霞 李雪明 宋天顺[1,2] 谢婧婧[1,2,3] LIU Haixia;LI Xueming;SONG Tianshun;XIE Jingjing(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China;State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing Tech University,Nanjing 210009,China;Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM),Nanjing 210009,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]南京工业大学材料化学国家重点实验室,江苏南京210009 [3]江苏省先进生物制造协同创新中心,江苏南京210009

出　　处：《生物加工过程》2019年第5期474-480,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21878150)

摘　　要：采用平板涂布研究电子介体中性红、亚甲基蓝和甲基紫精对大肠杆菌BL21(DE3)细胞生长的抑制情况。通过微生物燃料电池技术(MFC)研究同一浓度下上述电子介体对大肠杆菌BL21(DE3)产电性能的影响。结果表明:甲基紫精对大肠杆菌BL21(DE3)的生长抑制最强,中性红对大肠杆菌BL21(DE3)的生长抑制作用较弱;添加亚甲基蓝对于大肠杆菌BL21(DE3)MFC产电性能的提高显著;添加0.06g/L亚甲基蓝对大肠杆菌BL21(DE3)MFC产电性能的加强效果最好。We studied the growth inhibition of Escherichia coli BL21(DE3) cells by neutral red,methylene blue and methyl viologen by plate coating.Subsequently,microbial fuel cell was used to study the effects of the common electron mediators on electrical performance of E.coli BL21(DE3) at the same concentration.Methyl viologen had the strongest growth inhibitory effect on E.coli BL21(DE3),whereas neutral red had a weaker growth inhibitory effect.The addition of methylene blue significantly improved the electrical performance of E.coli.E.coli BL21(DE3) had the best electrical performance in the microbial fuel cell when 0.06 g/L methylene blue was added.

关 键 词：微生物燃料电池 大肠杆菌BL21(DE3) 电子介体 中性红 亚甲基蓝 甲基紫精 产电性能 

分 类 号：X382[环境科学与工程—环境工程]