河西走廊地区幽门螺杆菌iceA、babA2基因分型与疾病关联性研究  

Relationship between genotypes of Helicobacter pylori iceA,babA2 and diseases in Hexi Corridor

作　　者：王猛[1] 韩俭[2] 周伟[3] 刘晔华[1] 张富花 穆红[1] WANG Meng;HAN Jian;ZHOU Wei;LIU Yehua;ZHANG Fuhua;MU Hong(Department of Laboratory,Tianjin First Central Hospital,Tianjin 300192,China;Institute of Pathogenic Biology,Lanzhou University,Lanzhou,Gansu 730000,China;Infectious Diseases Laboratory,the First Hospital of Lanzhou University,Lanzhou,Gansu 730000,China;Department of Gastroenterology,the Second People′s Hospital of Gansu Province,Lanzhou,Gansu 730000,China)

机构地区：[1]天津市第一中心医院检验科,天津300192 [2]兰州大学病原生物研究所,兰州730000 [3]兰州大学第一医院感染科研究室,兰州730000 [4]甘肃省第二人民医院消化内科,兰州730000

出　　处：《重庆医学》2019年第18期3126-3129,3133,共5页Chongqing medicine

基　　金：天津市临床重点学科(实验诊断学)建设项目资助课题(津卫科教2018-320);天津市第一中心医院春风基金项目(院CF201817)

摘　　要：目的探讨胃癌高发区河西走廊地区患者幽门螺杆菌(Hp)iceA和babA基因型的分布及其与消化道疾病相关因素的关系。方法收集甘肃省第二人民医院、张掖市人民医院、武威市肿瘤医院及金川公司职工医院82例胃镜检查患者的胃黏膜标本、从胃黏膜中分离并培养Hp菌株,设计引物进行PCR扩增及基因型判定,通过病例资料整理,分析Hp菌株中iceA和babA2基因不同基因型与患者临床病理类型、性别和年龄的关系。结果分离Hp菌株并成功扩增73份目的基因,其中HpiceA1型和2型的构成比分别为76.71%(56/73)和23.29%(17/73),babA2基因型为38.36%(28/73)。HpiceA1型在浅表性胃炎、萎缩性胃炎、消化性溃疡组的患者中的感染率均明显高于iceA2型(P<0.05),而胃癌组患者HpiceA1型感染率均明显低于iceA2型(P<0.05);HpiceA1型在20~<41岁、41~<61岁年龄组的患者中的感染率均显著高于iceA2型(P<0.05),不同性别患者间iceA基因型Hp感染率比较差异无统计学意义(P>0.05)。消化性溃疡、胃癌患者中babA2型感染率明显高于其他病理组(P<0.05);不同年龄及性别患者间babA2基因型Hp感染率比较,差异无统计学意义(P>0.05)。结论河西走廊地区Hp菌株iceA基因均为阳性并且以1型为主,其分布与患者的疾病类型、年龄相关;babA2基因型感染多与消化性溃疡和胃癌有关。Objective To investigate the distribution of iceA and babA genotypes of Helicobacter pylori (Hp) and its relationship with digestive tract disease in Hexi Corridor with high incidence of gastric cancer. Methods The gastric mucosa samples from 82 patients underwent gastroscopy in the regional hospitals in Hexi Corridor.Hp strains were isolated and cultured.The primers were designed for PCR amplification and genotype determination.According to the case information,the relationship between different genotype of iceA and babA2 in Hp strains and patients clinical pathological type,gender and age were analyzed by statistics. Results The strains isolated and 73 target genes were successfully amplified.Type 1 and type 2 of iceA were 76.71 %(56/73) and 23.29%(17/73),while genotype babA2 accounted for 38.36%(28/73).The infection of genotype iceA1 was significantly higher than iceA2 in patients with superficial gastritis,atrophic gastritis and peptic ulcer (P<0.05).The infection rate of Hp iceA1 was significantly lower than that of iceA2.The infection of genotype iceA1 was significantly higher than iceA2 in patients of 20-<41 and 41-<61 years old.There was no significant difference in the rate of iceA infection among patients from different genders (P > 0.05 ).The infection rate of babA2 in patients with peptic ulcer and gastric cancer was significantly higher than that in other pathological groups (P<0.05).There was no significant difference in the rate of iceA and babA2 genotype infection among patients in different ages and genders (P >0.05). Conclusion The Hp stains from Hexi Corridor were all iceA genotype positive,and iceA1 was the main genotype.The distribution of iceA has been associated with the disease types and ages.And the infection of babA2 genotype has been associated with peptic ulcer and gastric cancer.

关 键 词：幽门螺杆菌 上皮细胞接触诱导基因 血型抗原Lewisb结合黏附基因A 河西走廊 

分 类 号：R735.2[医药卫生—肿瘤]