LINC00052通过微小RNA-330-3p/RCAN1轴对肝癌细胞增殖和迁移的影响  

Effects of LINC00052 targeting microRNA-330-3p/RCAN1 axis on proliferation and migration of hepatocellular carcinoma

作　　者：王哲[1] 宋展[2] 万春[1] 乔兵兵[3] Wang Zhe;Song Zhan;Wan Chun;Qiao Bingbing(Department of Liver Surgery,Nanyang Central Hospital,Nanyang 473003,China;Department of General Surgery,Nanyang Central Hospital,Nanyang 473003,China;Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]南阳市中心医院肝脏外科,473003 [2]南阳市中心医院普外科,473003 [3]郑州大学第一附属医院肝胆胰外科,450052

出　　处：《中华实验外科杂志》2019年第9期1583-1586,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察LINC00052靶向微小RNA(miRNA,miR)-330-3 p/RCAN1对肝癌增殖和迁移的影响.方法收集正常肝癌组织和癌旁组织各100例,采用荧光定量PCR分析LINC00052和miR-330-3p表达水平.采用生物信息学和双荧光素酶报告基因分析LINC00052和miR-330-3p间的关系以及miR-57的靶基因;在肝癌细胞株MHCC97H中构建LINC00052敲降细胞株和miR-330-3p过表达细胞株,采用EDU法和Transwell实验肝癌细胞的增殖和迁移能力.结果与肝癌癌旁组织LINC00052和miR-330-3p[(1.01±0.21),(1.12±0.21)]比较,肝癌组织LINC00052表达水平(0.41±0.13)显著下降,miR-330-3p表达水平(2.37±0.20)显著下调,差异均有统计学意义(t=3.691、3.110,P<0.05)].生物信息学和双荧光素酶分析显示LINC00052能与miR-330-3p互补结合,miR-330-3p调节着RCAN1蛋白表达.与Linc-contrtol组细胞[(67.43±9.32)、(120.32±11.32)]比较,LINC00052组肝癌细胞的增殖和迁移[(29.44±6.81),(73.41±9.76)]明显抑制,差异均有统计学意义(t=4.198、3.109,P<0.05).与miR-control组[(69.33±10.98)、(130.22.33±13.19)]比较,miR-330-3p KD组肝癌细胞增殖和迁移[(35.32±7.12),(63.39±10.32)]显著抑制,差异均有统计学意义(t=3.091、4.011,P<0.05).与LINC-contrtol组和miR-control组肝癌细胞RCAN1表达水平[(0.51±0.13)、(0.49±0.10)]比较,LINC00052组和miR-330-3p KD组细胞RCAN1蛋白表达水平[(1.23±0.23)、(1.03±0.12)]显著增加,差异有统计学意义(t=4.339、4.211,P<0.05).结论LINC00052通过靶向作用于miR-330-3p/RCAN1进而调节着肝癌细胞的增殖和迁移.Objective To investigate Effects of LINC00052 targeting microRNA(miRNA,miR)-330-3p/RCAN1 axis on proliferation and migration of hepatocellular carcinoma.Methods 100 cases of normal adjacent tissues and 100 cases of hepatocellular carcinoma tissues were collected.The expression levels of LINC00052 and miR-330-3p were analyzed by fluorescence quantitative PCR.Bioinformatics was used to analyze the relationship between LINC00052 and miR-330-3p and target genes;knockdown cell line of miR-330-3p and LINC00052 overexpression cell line were constructed in MHCC97H cell line.The proliferation and migration ability were analyzed by EDU staining and Transwell.Results Compared with LINC00052 and Mi-330-3p in adjacent tissues of hepatocellular carcinoma[(1.01±0.21),(1.12±0.21)],the expression level of LINC00052 in hepatocellular carcinoma significantly decreased(0.41±0.13)and the expression level of Mi-330-3p significantly decreased(2.37±0.20)(t=3.691,3.110,P<0.05).Bioinformatics and double luciferase analysis showed that LINC00052 could complement and bind to microRNA-330-3p and microRNA-330-3p regulated the expression of RCAN1 protein.Compared with LINC-contrtol group[(67.43±9.32),(120.32±11.32)],the proliferation and migration of hepatocellular carcinoma cells in LINC00052 group[(29.44±6.81),(73.4±9.76)]significantly inhibited and the difference was statistically significant(t=4.198,3.109,P<0.05).Compared with the microRNA-control group[(69.33±10.98),(130.22.33±13.19)],the proliferation and migration of hepatocellular carcinoma cells in microRNA-330-3p KD group[(35.32±7.12),(63.39±10.32)]significantly inhibited(t=3.091,4.011,P<0.05).Compared with the expression levels of RCAN1 in LINC-contrtol group and miR-control group[(0.51±0.13),(0.49±0.10)],the expression levels of RCAN1 in LINC00052 group and miR-330-3p KD group(1.23±0.23),(1.03±0.12)]significantly increased(t=4.339,P<0.05;t=4.211,P<0.05).Conclusion LINC00052 can regulate the proliferation and migration of hepatocellular carcinoma cells by targeting m

关 键 词：LINC00052 微小RNA-330-3p RCAN1 肝癌细胞 增殖 迁移 

分 类 号：R735.7[医药卫生—肿瘤]