微小RNA-877对胃癌细胞增殖及侵袭的影响  

Effects of microRNA-877 on proliferation and invasion of gastric cancer cells

作　　者：郭魁元[1] 闫军浩 罗昭锋 杨战锋[1] 吴万庆[1] 郭晓磊[1] 傅聿铭[1] 崔小兵[1] Guo Kuiyuan;Yan Junhao;Luo Zhaofeng;Yang Zhanfeng;Wu Wanqing;Guo Xiaolei;Fu Yuming;Cui Xiaobing(Department of General Surgery,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第五附属医院普外科,450052

出　　处：《中华实验外科杂志》2019年第9期1587-1589,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察微小RNA(miRNA,miR)-877对SGC7901胃癌细胞增殖及侵袭的影响.方法采用实时定量聚合酶链反应(Real-time PCR)检测miR-877在胃癌细胞株(SGC7901、MGC803、MKN-45、BSG823、HGC-27、AGS)及正常人胃黏膜上皮细胞株(GES-1)中的相对表达量.合成miR-877激动剂(miR-877 mimics),应用LipofectamineTM2000将miR-877 mimics及阴性对照转染SGC7901胃癌细胞,细胞计数试剂盒(CCK-8)检测细胞增殖能力,Transwell法检测细胞侵袭能力,蛋白质印迹法(Western blot)检测磷脂酰肌醇-3-激酶(PI3K)、蛋白激酶B(PKB,又称Akt)、磷酸化Akt(p-Akt)蛋白表达.应用GraphPad Prism 7.0统计软件分析,两样本均数间的比较采用Studentt检验.结果miR-877在胃癌细胞株SGC7901(0.21±0.05),MGC803(0.63±0.11),MKN-45(0.35±0.07),BSG823(0.49±0.08),HGC-27(0.75±0.10),AGS(0.43±0.07)中的相对表达量明显低于GES-1(1.09±0.13)细胞(P均<0.05).MiR-877 mimics组(0.27±0.05)细胞中miR-877的相对表达量低于阴性对照组(1.06±0.11,t=11.690,P<0.01).CCK-8实验结果显示,72 h后miR-877mimics组吸光度值(1.59±0.09)低于阴性对照组(2.28±0.11,t=4.746,P<0.01).Transwell实验结果显示,miR-877 mimics组(43.60±17.30)细胞侵袭数量显著低于阴性对照组(129.35±16.70,t=8.148,P<0.01).Western blot结果显示miR-877 mimics组细胞中PI3K、Akt、p-Akt的表达量低于阴性对照组(P均<0.01).结论miR-877可抑制胃癌细胞增殖与侵袭,这一作用与抑制PI3K/Akt信号通路有关.Objective To investigate the effects of microRNA(miRNA,miR)-877 on proliferation and invasion of SGC7901 gastric cancer cells.Methods The relative expression level of miR-877 in gastric cancer cell lines(SGC7901,MGC803,MKN-45,BSG823,HGC-27,AGS)and normal gastric cell line(GES-1)were measured by real-time quantitative polymerase chain reaction(Real-time PCR).MiR-877 agonist(miR-877 mimics)was synthesized.MiR-877 mimics and miR-877 negative control were transfected into SGC7901 cells by liposome Lipofectamine^TM 2000.Cell proliferation ability was detected by cell counting kit-8(CCK-8)assay.Cell invasion ability was evaluated by Transwell method.The expression of phosphatidylinositol-3-kinase(PI3K),protein kinase B(PKB,also called Akt),phosphorylated Akt(p-Akt)were detected by Western blotting.Results The relative expression level of miR-877 was significantly lower in gastric cancer cell lines SGC7901(0.21±0.05),MGC803(0.63±0.11),MKN-45(0.35±0.07),BSG823(0.49±0.08),HGC-27(0.75±0.10),AGS(0.43±0.07)than that in GSE-1 cells(1.09±0.13,all P<0.05).The relative expression level of miR-877 in miR-877 mimics group(0.27±0.05)was significantly lower than that in negative control group(1.06±0.11,t=11.690,P<0.01).The results of CCK-8 assay showed that the absorbance value of miR-877 mimics group(1.59±0.09)was significantly lower than that of negative control group(2.28±0.11,t=4.746,P<0.01)after culturing 72 h.The results of Transwell assay revealed that cell invasion number in miR-877 mimics group(43.60±17.30)was significantly lower than that in negative control group(129.35±16.70,t=8.148,P<0.01).The results of Western blotting manifested that the expression level of PI3K,Akt,p-Akt in miR-877 mimics group were significantly lower than that in negative control group(all P<0.01).Conclusion MiR-877 could inhibit the proliferation and invasion of gastric cancer cells,which is involved in suppressing PI3K/AKt signaling pathway.

关 键 词：胃癌 微小RNA-877 增殖 侵袭 磷脂酰肌醇-3-激酶/蛋白激酶B信号通路 

分 类 号：R735.2[医药卫生—肿瘤]