微小RNA-10a靶向MTMR3对胶质瘤细胞生长和迁移的影响  

Effect of microRNA-10a targeting MTMR3 on growth and migration of glioma cells

作　　者：万学锋 李勇[2] 庞留勇[2] 刘献志[3] Wan Xuefeng;Li Yong;Pang Liuyong;Liu Xianzhi(Dpartment of Neurosurgery,the Zhumadian City Center Hospital,Henan 463000,China;Department of Nursing,HuangHuai University,Henan 463000,China;Department of Neurosurgery,the First Affiliated Hospital of Zhengzhou University,Henan 450000,China)

机构地区：[1]驻马店市中心医院神经外科,463000 [2]黄淮学院护理学院,河南驻马店463000 [3]郑州大学第一附属医院神经外科,450000

出　　处：《中华实验外科杂志》2019年第9期1611-1614,共4页Chinese Journal of Experimental Surgery

基　　金：河南省科技计划项目(18210241002).

摘　　要：目的探讨微小RNA(miRNA,miR)-10a对胶质瘤细胞生长和迁移的影响及分子机制.方法收集脑胶质瘤和癌旁组织,采用荧光定量聚合酶链反应(PCR)分析miR-10a的表达水平.在U251脑胶质瘤细胞株中构建miR-10a过表达细胞株(实验组)和对照细胞株(对照组),采用细胞计数试剂盒(CCK-8)试剂盒和Transwell分析不同处理的脑胶质瘤细胞的增殖和迁移能力.采用生物信息学和双荧光素酶报告基因分析miR-10a与靶基因的关系;并在miR-10a过表达细胞株过表达靶基因,分析细胞的增殖和迁移能力.采用t检验分析两组各指标差异.结果与脑胶质瘤癌旁组织(1.01±0.19)比较,脑胶质瘤组织中miR-10a表达水平(2.38±0.28)显著上调,差异有统计学意义(t=3.918,P<0.05).实验组细胞增殖能力较对照组显著增加,差异有统计学意义(F=2.991,P<0.05).与对照组(48.67±13.42)个比较,实验组肿瘤细胞迁移能力(128.32±19.32)个显著增加,差异有统计学意义(t=4.109,P<0.05).生物信息学分析显示MTMR3是miR-10a的靶基因.与对照组细胞(1.19±0.21)比较,实验组细胞MTMR3蛋白的表达水平显著下调(0.21±0.09),差异有统计学意义(t=3.819,P<0.05).在补偿实验中,与过表达对照载体的细胞比较,过表达MTMR3蛋白则显著抑制了脑胶质瘤细胞的增殖和迁移,差异有统计学意义(P<0.05).结论miR-10a在脑胶质瘤中呈现高表达,并通过调节MTMR3蛋白调控着脑胶质瘤细胞的增殖和迁移.Objective To investigate the effect of microRNA(miRNA,miR)-10a on the growth and migration of glioma cells and its molecular mechanism.Methods The expression levels of miR-10a in glioma and adjacent tissues were analyzed by fluorescence quantitative polymerase chain reaction(PCR).miR-10a overexpression cell line(experimental group)and control cell line(control group)were constructed in U251 glioma cell line.The proliferation and migration ability of glioma cells in control group and experimental group were determined by CCK-8 kit and Transwell.The target genes of miR-10a were analyzed by Bioinformatics and double luciferase reporter genes.The proliferation and migration ability of cells in experimental group cells that overexpressed target genes were analyzed.The difference between two groups were analyzed by t test.Results Compared with glioma adjacent tissues(1.01±0.19),the expression of miR-10a in glioma tissues(2.38±0.28)(t=3.918,P<0.05).The cell proliferation ability in the experimental group was significantly higher than that of the control group(F=2.991,P<0.05).Compared with the control group(48.67±13.42),the migration ability of tumor cells in the experimental group(128.32±19.32)increased significantly(t=4.109,P<0.05).Bioinformatics analysis showed that MTMR3 was the target gene of miR-10a.Compared with control cells(1.19±0.21),the expression level of MTMR3 protein in experimental cells(0.21±0.09)significantly decreased(t=3.819,P<0.05).Overexpression of MTMR3 protein significantly inhibited the proliferation and migration of glioma cells in miR-10a overexpression cells compared with those in overexpression control vectors(P<0.05).Conclusion MiR-10a is highly expressed in glioma and regulates the proliferation and migration of glioma cells by targeting MTMR3 protein.

关 键 词：微小RNA-10a MTMR3 脑胶质瘤 增殖 迁移 

分 类 号：R739.41[医药卫生—肿瘤]