C/EBP同源蛋白在紫檀芪抗人肺鳞癌Calu-1细胞中作用机制的研究  

Pterostilbene inhibits lung squamous cell carcinoma Calu-1 cell via activation of C/EBP homologous protein

作　　者：庞赛楠 孙大为[1] 王俊峰[1] 任凤海 卜建龙[1] 孔祥龙 朱开彬 徐世东[1] Pang Sainan;Sun Dawei;Wang Junfeng;Ren Fenghai;Bu Jianlong;Kong Xianglong;Zhu Kaibin;Xu Shidong(Department of Thoracic Surgery,Harbin Medical University Cancer Hospital,Heilongjiang Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属肿瘤医院胸外科

出　　处：《现代肿瘤医学》2019年第19期3365-3369,共5页Journal of Modern Oncology

基　　金：黑龙江省卫生计生委科研课题(编号:2016-099)

摘　　要：目的:探讨紫檀芪(PT)抗人肺鳞癌Calu-1细胞作用机制及内质网应激(ERS)相关蛋白C/EBP同源蛋白(CHOP)在其中的作用。方法:实验分为对照组、PT组(15、30、45μmol/L),分别检测各组Calu-1细胞活力值、凋亡率、ROS含量及Caspase-3活性,以及CHOP蛋白和凋亡相关蛋白(Bcl-2、Bax)含量的变化。CHOP小干扰RNA(siRNA)特异性抑制CHOP蛋白表达后,PT(0、30μmol/L)处理细胞24h,重复上述检测。结果:PT有效抑制Calu-1细胞增殖,促进凋亡(P<0.05),显著增加ROS含量及Caspase-3活性(P<0.05),并呈浓度依赖效应;Western blot检测结果显示PT上调CHOP及Bax的表达(P<0.05),抑制Bcl-2的表达(P<0.05);CHOPsiRNA转染显著抑制CHOP表达,同时抑制PT对Calu-1细胞的上调CHOP蛋白表达和促凋亡作用(P<0.05)。结论:紫檀芪可有效抑制肺鳞癌Calu-1细胞活力、促进细胞凋亡,此作用可能与激活内质网应激相关蛋白CHOP有关。Objective:To investigate the anticancer effect of pterostilbene (PT) on human lung squamous cell carcinoma Calu-1 cell line and explore the role of endoplasmic reticulum stress (ERS) signaling related protein,C/EBP homologous protein (CHOP),in this process.Methods:Calu-1 cells were divided into the Control group and PT groups (15,30,45 μmol/L).The cell viability rate,the apoptotic rate and the activity of Caspase-3 and ROS level of cells were respectively detected.Western blot was used to detect the expression of CHOP and apoptosis related proteins (Bax and Bcl-2) in Calu-1 cells.Moreover,small interfering RNA (siRNA) of CHOP transfection was used to block the CHOP expression in Calu-1 cells,then PT treatments (0,30 μmol/L) were implemented for further detection as mentioned in 24 hours.Results:PT treatments resulted in a significant reduction in cell viability and upregulation of cell apoptosis and production of Caspase-3 and ROS in a dose-dependent manner.Western blot analysis showed that PT treatment resulted in a significant increase of CHOP and Bax expression,and decrease of Bcl-2 expression.Moreover,inhibition of CHOP by siRNA decreased the anticancer role of PT on Calu-1 cells.Conclusion:PT treatment can effectively inhibit the proliferation and enhance the apoptosis of Calu-1 cells,which may be related to the activation of CHOP.

关 键 词：紫檀芪 肺鳞癌 C/EBP同源蛋白 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]