赖氨酸芽孢杆菌He14发酵产物抗真菌活性的初步研究  

Antifungal activity of fermentation broth of Lysinibacillus fusiformis He14

作　　者：郑雯 杨兴变[2] 康冀川[1,2] 李洪庆[2,3] ZHENG Wen;YANG Xingbian;KANG Jichuan;LI Hongqing(School of Pharmaceutical Sciences,Guizhou University,Guiyang 550025,China;Engineering Research Center of the Utilization for Characteristic Bio-Pharmaceutical Resources in Southwest,Ministry of Education,Guizhou University,Guiyang 550025,China;School of Chemical Engineering,Guizhou Minzu University,Guiyang 550025,China)

机构地区：[1]贵州大学药学院,贵州贵阳550025 [2]贵州大学,西南特色药用生物资源开发利用教育部工程研究中心,贵州贵阳550025 [3]贵州民族大学化学工程学院,贵州贵阳550025

出　　处：《食品与发酵工业》2019年第18期22-26,共5页Food and Fermentation Industries

基　　金：教育部留学回国人员科研启动基金(批准文号:教外司留[2008]890号);贵州大学教育部重点实验室(工程中心)开放课题基金(合同编号:GZUKEY20160703)

摘　　要：初步研究赖氨酸芽孢杆菌(Lysinibacillus fusiformis)He14发酵产物的抗真菌活性。采用生长速率法研究He14发酵液对8株病原真菌的拮抗作用。对抑真菌活性成分进行稳定性试验。考察萃取、沉淀和大孔吸附树脂对发酵液活性成分的富集情况。采用硅胶柱层析对活性成分进行初步纯化。菌株He14发酵液对8株病原真菌的生长均有抑制作用。抑真菌活性成分对高温、强酸强碱及紫外有一定稳定性。筛选到LX-60大孔吸附树脂对活性物质的富集效果最好。活性粗产物经硅胶柱层析得到3个混合组分,对辣椒灰霉病菌、马铃薯晚疫病菌、马铃薯早疫病菌、水稻纹枯病菌和须毛癣菌有不同程度的抑制作用,其中组分Ⅱ对马铃薯晚疫病菌抑制效果较好。赖氨酸芽孢杆菌He14在拮抗病原真菌方面有许多优良性状,研究结果为该菌在生物防治及医药方面的应用提供了实验基础。The antifungal activity of fermentation broth of Lysinibacillus fusiformis He14 was analyzed,and eight pathogenic fungi were selected in this study.The stabilities of the antifungal components during extraction,precipitation and macroporous resin adsorption processes were investigated,respectively.It was found that the fermentation broth of strain He14 had inhibitory effects on all the eight pathogenic fungi and the bioactive substances were stable under extreme conditions of temperature,pH and UV.Moreover,LX-60 macroporous resin showed the best capability to adsorb and desorb bioactive components and three mixed components obtained through silica gel column chromatography showed inhibitory effects against Botrytis cinerea,Phytophthora infestans,Alternaria solani,Thanatephorus cucumeris and Trichophyton mentagrophytes.ComponentⅡexhibited the highest inhibition effect on P.infestans.It was concluded that L.fusiformis He14 has excellent traits in antipathogenic fungus,which provides an experimental basis for the application of L.fusiformis in the fields of biological control and medicine.

关 键 词：赖氨酸芽孢杆菌 抗真菌组分 稳定性 活性富集 活性组分 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]