大叶补血草根茎总黄酮的提取和抗氧化活性研究  

Study on Extraction and Antioxidant Activity of Total Flavonoids from the Root of Limonium gmelinii (Wiid)Kuntze

作　　者：陈梅[1] 葛鹏 何春霞[1] CHEN Mei;GE Peng;HE Chun-xia(Department of Petroleum and Chemical Engineering,Xinjiang Applied Vocational Technical College,Kuitun,Xinjiang 833200)

机构地区：[1]新疆应用职业技术学院石油与化学工程系

出　　处：《安徽农业科学》2019年第19期190-192,共3页Journal of Anhui Agricultural Sciences

基　　金：新疆维吾尔自治区自然科学基金项目(201318101-18)

摘　　要：[目的]研究大叶补血草根茎总黄酮提取物的抗氧化活性。[方法]采用超声提取考察不同产地不同溶剂提取的大叶补血草总黄酮含量;采用Fenton反应体系、改良的邻苯三酚自氧化体系和硫代巴比妥酸(TBA)法分别测定大叶补血草总黄酮对羟自由基、超氧阴离子自由基和脂质过氧化的抑制作用。[结果]醇提总黄酮的产率高于水提取,产于阿勒泰的大叶补血草总黄酮含量略高;抗氧化试验结果显示,大叶补血草总黄酮对·OH、O2·-的产生和脂质过氧化均具有抑制作用,采于阿勒泰地区的大叶补血草中总黄酮的抗氧化活性略高于伊犁地区的,而醇提取液的抗氧化活性明显大于水提液的活性,当浓度达到0.83mg/mL时,醇提取液对羟自由基(·OH)的清除率达98.9%。[结论]大叶补血草根部总黄酮含量可观,作为抗氧化剂具有很好的开发应用价值和前景。[Objective]The research aimed to study the antioxidant activity of the total flavonoid extract from the rhizome of Limonium gmelinii.[Method]The content of total flavonoids in Limonium gmelinii from different areas was investigated by ultrasonic extraction with different solvents.Fenton reaction,improved pyrogallol autoxidation system and TBA method were used to measure the inhibition effect of·OH,O 2·-and lipid peroxidation by total flavonoids.[Result]The yield of total flavonoids from alcohol extraction was higher than that from water extraction,and the content of total flavonoids from ALTGL was slightly higher;the results of anti-oxidation experiments showed that the total flavonoids possessed certain inhibition effect of·OH,O 2·-and lipid peroxidation,the antioxidant activity of ALTGL slightly higher than KTGL,and that of alcohol extract was significantly greater than water extract,the clearance rate of hydroxyl free radical by alcohol extract was reached 98.9%when the concentration of 0.83 mg/mL.[Conclusion]The total flavonoids content of Limonium gmelinii root is considerable,and it has great development application value and prospect as an antioxidant.

关 键 词：大叶补血草 根茎 总黄酮 提取 抗氧化活性 

分 类 号：R282.71[医药卫生—中药学]