基于血脑屏障靶向多肽的脑神经分析化学研究进展  

Targeted Analysis of Central Nervous System Using Blood-Brain Barrier Shuttle Peptides

作　　者：黄嫣嫣[1,2] 赵睿 HUANG Yan-Yan;ZHAO Rui(Beijing National Laboratory for Molecular Sciences,CAS Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]北京分子科学国家研究中心,中国科学院化学研究所,中科院活体分析化学重点实验室,北京100190 [2]中国科学院大学,北京100049

出　　处：《分析化学》2019年第10期1629-1638,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21874141,91853103,21675161,21705154);中国科学院青年创新促进会项目(No.2015027)资助~~

摘　　要：将用于分析检测的分子或材料透过血脑屏障(Blood-brain barrier,BBB),使其高效靶向中枢神经系统,是实现原位、在体脑神经化学分析亟需解决的问题。多肽作为内源性生理活性物质,具有模块化的结构,不仅设计性强、构象可预测,而且利用固相合成方法可高效、低干扰地获得目标序列。以小分子多肽为穿梭工具,可将荧光分子、造影剂、纳米颗粒等高效、快速地转运到目标位置,为脑科学研究及相关疾病的检测、分子机制研究提供新方法和新技术。本文围绕BBB和多肽的特点,从BBB穿透途径和靶向多肽设计筛选方法出发,对近年来基于多肽(Peptide shuttles)的BBB靶向分析的研究进展进行了总结和评述,期望从分子、细胞到活体的角度为脑神经分析化学研究提供参考和借鉴。Targeted delivery of molecules and materials across blood-brain barrier(BBB)is critical for in-situ and real-time analysis of central nervous system,which is also a challenge for analytical chemistry.As a kind of endogenous bioactive species,peptides with modulate structure can be designed with predictable conformations and chemically synthesized with high efficiency and purity.Peptides can act as BBB shuttles to transport fluorescent dyes,contrast agents and nanomaterial to target tissues,providing new tools and analytical methods for brain science,disease detection and molecular mechanism investigation.This review summarizes recent advances in analytical applications of BBB shuttle peptides for studying central nervous system.Introduction on BBB,peptides as well as design and screening approaches of BBB shuttle peptides is also included.

关 键 词：血脑屏障 多肽 靶向分析 亲和筛选 评述 

分 类 号：O652[理学—分析化学] R446.1[理学—化学]