miR-143-3p可能经MAPK7途径抑制食管癌细胞的增殖、迁移与侵袭  

Effects of miR-143-3p on proliferation,migration and invasion of esophageal cancer cells through MAPK7 pathway

作　　者：薛亚军[1] 杜雅彦 黄文华 耿涛[1] 魏育涛[1] 胡建明[1] XUE Ya-Jun;DU Ya-Yan;HUANG Wen-Hua;GENG Tao;WEI Yu-Tao;HU Jian-Ming(School of Medicine,Shihezi University,Shihezi 832000,China)

机构地区：[1]新疆石河子大学医学院

出　　处：《中国免疫学杂志》2019年第18期2177-2180,2186,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81760428)资助项目

摘　　要：目的:探讨miR-143-3p通过MAPK7途径对抑制食管癌细胞增殖、迁移和侵袭的影响。方法:采用脂质体转染法转染miR-143-3p拟似物(拟似组)、阻遏物(阻遏组)和阴性对照物(阴性组)至ECA109细胞,荧光实时定量PCR法检测细胞内miR-143-3p含量,MTT法检测细胞增殖活性,Transwell小室法检测miR-143-3p细胞侵袭,观察裸鼠成瘤质量和瘤内MAPK7表达。结果:miRNA-143-3p表达12、24、48、72h时空白组和阴性组细胞内miRNA-143-3p表达相比较差异无统计学意义(P>0.05),均低于阻遏组,高于拟似组,差异均有统计学意义(P<0.05);空白组和阴性组24、48、72h时ECA109细胞增殖水平相比较差异均无统计学意义(P>0.05),均高于拟似组,低于阻遏组,差异均有统计学意义(P<0.05);空白组和阴性组ECA109细胞侵袭能力相比较差异均无统计学意义(P>0.05),均高于拟似组,低于阻遏组,差异均有统计学意义(P<0.05);空白组和阴性组裸鼠瘤重量均高于拟似组,低于阻遏组,差异均有统计学意义(P<0.05),空白组和阴性组裸鼠瘤重量相比较差异无统计学意义(P>0.05);空白组和阴性组裸鼠瘤组织MAPK7蛋白表达水平相比较差异无统计学意义(P>0.05),均高于拟似组,低于阻遏组,差异均有统计学意义(P<0.05)。结论:miRNA-143-3p表达异常是影响食管癌增殖侵袭的重要因素,MAPK7信号通路可能是其发挥作用的重要途径,详细机制尚有待进一步研究探讨。Objective:To investigate the effect of miR-143-3p on proliferation,migration and invasion of ductal carcinoma cells through MAPK7 pathway.Methods:miR-143-3p mimicry,repressor and negative control group were transfected into ECA109 cells by liposome transfection.miR-143-3p in the cells was quantified by real-time fluorescence quantitative PCR;proliferation and invasion of miR-143-3p transfected ECA109 cells was detected by MTT and Transwell cell assay,respectively.Tumor mass and MAPK7 expression were measured in nude mice.Results:The expression of miRNA-143-3p in the blank group and the negative group was lower than that of the repressor group and higher than that of the imitated group at 12 h,24 h,48 h and 72 h,and the difference was statistically significant(P<0.05).The proliferation level and invasive ability of ECA109 cells in blank group and negative group at 24 h,48 h and 72 h were higher than those in similar group,but lower than those in repression group(P<0.05).The tumor weight of nude mice in blank group and negative group was higher than that of imitated group and lower than that of imitated group(P<0.05).There was no significant difference in tumor weight between blank group and negative group(P>0.05).There was no significant difference in the expression of MAPK7 protein between the blank group and the negative group(P>0.05),which was higher than the similar group and lower than the repressive group(P<0.05).Conclusion:The abnormal expression of miRNA-143-3p is an important factor affecting the proliferation and invasion of esophageal cancer.MAPK7 signaling pathway may be an important way to play its role.The detailed mechanism needs further study.

关 键 词：食管癌 微小RNA 丝裂酶原活化蛋白激酶 

分 类 号：R735.1[医药卫生—肿瘤]