粉单竹竹叶提取物的抑菌活性研究  

Antibacterial Research on the Bamboo Leaf Extracts from Bambusa cerosissima McClure

作　　者：祁红兵 宋军霞 张志敏 QI Hongbing;SONG Junxia;ZHANG Zhimin(Life Sciences and Technology School, Lingnan Normal University, Zhanjiang 524048, China;Physical Sciences and Technology School, Lingnan Normal University, Zhanjiang 524048, China)

机构地区：[1]岭南师范学院生命科学与技术学院,广东湛江524048 [2]岭南师范学院物理科学与技术学院,广东湛江524048

出　　处：《云南农业大学学报（自然科学版）》2019年第5期906-910,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家星火计划项目(2015GA780051);产业链协同创新类公共服务平台项目(湛海创2017C8B2);广东省岭南师范学院校内专项项目(2L1311)

摘　　要：【目的】研究粉单竹竹叶提取物的抗菌活性及其稳定性,为其应用提供参考。【方法】采用抑菌圈法研究系统分离得到的粉单竹竹叶提取物对金黄色葡萄球菌、大肠杆菌、酿酒酵母和黄曲霉等常见微生物的抑菌活性;确定最低抑菌质量浓度;考察温度、pH、紫外线和化学药剂等因素对提取液抗菌活性的影响。【结果】竹叶提取物系统分离各部分对4种菌有不同的抑制作用,其中正丁醇萃取物对金黄色葡萄球菌的抑菌效果最好,最小抑菌质量浓度是1.67×10^-1 mg/mL;石油醚萃取物和萃余水相物对大肠杆菌的抑制作用最好,最小抑菌质量浓度分别为1.38×10^-3 mg/mL和3.17×10^-3 mg/mL;正丁醇萃取物和萃余水相物对酿酒酵母的抑菌效果最好,最小抑菌质量浓度分别为1.67×10^-2 mg/mL和3.17×10^-2 mg/mL;只有氯仿萃取物对黄曲霉有抑菌作用。除加入Tween 20会明显增加抑菌活性外,各外界条件对竹叶提取物的抑菌性能影响较小。【结论】粉单竹竹叶提取物有较好的抑菌活性,且竹叶提取物的生物活性比较稳定,有较好的应用前景。[Purpose]The antibacterial activity and stability of bamboo leaf extracts from Bambusa cerosissima McClure were studied to provide references for their application.[Method]Using B. cerosissima as the material and distilled water, ethanol and petroleum ether, chloroform, ethyl acetate and N-butanol as the solvents, the antibacterial effects of bamboo leaf extracts on Staphylococcus aureus, Escherichia coli, Saccharomyces cerevisiae and Aspergillus flavus were studied by the method of bacteriostasis circle. The minimum bacteriostasis mass concentration was determined. The effects of temperature, pH, ultraviolet radiation and chemicals on the antibacterial activity of the extracts were also studied.[Results]Five bamboo leaf extract systems had different inhibitory effects on four kinds of bacteria, among which the N-butanol extract had the best inhibitory effect on S. aureus with the minimum inhibitory mass concentration of 1.67×10^-1 mg/mL. The petroleum ether extract and water soluble extract had the best inhibitory effect on E. coli with the minimum inhibitory mass concentrations of 1.38×10^-3 mg/mL and 3.17×10^-3 mg/mL, respectively. The butanol extract and water soluble extract had the best bacteriostatic effect on S. cerevisiae with the minimum bacteriostatic mass concentrations of 1.67×10^-2 mg/mL and 3.17×10^-2 mg/mL, respectively, and only the trichloromethane extract had bacteriostatic effect on A. flavus. In addition to Tween 20, the biological activity of bamboo leaf extract is stable for a variety of external conditions.[Conclusion]The extract of bamboo leaves has better bacteriostatic activity, and the biological activity of bamboo leaf extract is relatively stable, so it has a good application prospect.

关 键 词：粉单竹 竹叶提取物 抑菌活性 

分 类 号：S482.292[农业科学—农药学]