鳖甲水溶性蛋白含量测定方法比较  

Comparison of Three Methods of Determination for Water Soluble Protein in Trionyx sinensis Carapace

作　　者：翟玲 夏雨 杨艳芳[1] 吴和珍[1] 刘焱文[1] 尤朋涛[1] Zhai Ling;Xia Yu;Yang Yanfang;Wu Hezhen;Liu Yanwen;You Pengtao(Provincial and the Ministry of Education Key Laboratory of Resource Science and Chinese Herbal Compound in Hubei University of Traditional Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学中药资源与中药化学湖北省重点实验室

出　　处：《亚太传统医药》2019年第9期66-69,共4页Asia-Pacific Traditional Medicine

基　　金：湖北省科技厅项目(2018CFB657)

摘　　要：目的:探究准确高效测定鳖甲水溶性蛋白含量的方法。方法:以水为溶剂超声提取鳖甲蛋白,通过考马斯亮蓝G-250染色法、BCA(Bicinchonininc Acid)法、紫外吸收(UV)法对提取液中蛋白含量进行测定,并与凯氏定氮法对比。结果:蛋白浓度在0.4~1.5 mg/mL范围内,与凯氏定氮法的测定结果间的偏差分别为考马斯亮蓝G-250染色法:33%~48%,BCA法:5%~16%,紫外吸收法:>96%。结论:BCA法测定结果较准确,快速,消耗样品少,更适于鳖甲水提液中蛋白含量的测定。Objective:To explore an accurate and effective method of the determination for water soluble protein inTrionyx sinensis Carapace.Methods:TheTrionyx sinensis Carapace was extracted by the ultrosonic method,and Coomassie brilliant blue G-250 assay,bicinchonininc acid assay,UV spectrophotometric and contras Kjeldahlt was used for quantify of water soluble protein.Results:within the concentration of 0.4-1.5 mg/mL,the results of determinate deviation were 33%-48% by Coomassie brilliant blue G-250 assay,5%-16% by bicinchonininc acid assay,and > 96% by UV absorption.Conclusion :The determination of water soluble protein content in water extract of Trionyx sinensis Carapace using bicinchonininc acid method is accurate < rapid,and less consumption of samples,so it is optimum for the determination of water soluble protein content in water extract of Trionyx sinensis Carapace.

关 键 词：鳖甲 蛋白提取 含量测定 方法比较 

分 类 号：R284.2[医药卫生—中药学]