外周血循环肿瘤细胞及其趋化因子受体CCR9与非小细胞肺癌的临床相关性  

Clinical study on the correlation of peripheral blood circulating tumor cells and their chemokine receptor CCR9 with non-small cell lung cancer

作　　者：林思阳 蒿艳蓉[2] 吕艳茹[2] 冯国生[2] 林辉[3] 李柏钧[3] 袁贤彬[2] 曹宇华[2] 侯恩存[4] 陆翔[5] LIN Si-yang;HAO Yan-rong;Lü Yan-ru;FENG Guo-sheng;LIN Hui;LI Bai-jun;YUAN Xian-bin;CAO Yu-hua;HOU En-cun;LU Xiang(Gradute School,Guangxi Medical University,Nanning 530021,Guangxi,China;Clinical Oncology Center,The People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi,China;Department of Cardiovascular & Thoracic Surgery,the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi,China;Department of Oncology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,Guangxi,China;Department of Oncology,the First People′s Hospital of Nanning,Nanning 530022,Guangxi,China)

机构地区：[1]广西医科大学研究生院,南宁530021 [2]广西壮族自治区人民医院临床肿瘤中心,南宁530021 [3]广西壮族自治区人民医院胸心血管外科·胸部外科,南宁530021 [4]广西中医药大学附属瑞康医院肿瘤科,南宁530011 [5]南宁市第一人民医院肿瘤科,南宁530022

出　　处：《医学研究生学报》2019年第9期948-953,共6页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81560383);广西重点研发计划(桂科AB16380226)

摘　　要：目的循环肿瘤细胞(CTCs)在多种肿瘤的临床应用中具有潜在的应用价值。文中旨在探讨CTCs及其趋化因子受体CCR9在非小细胞肺癌(NSCLC)肿瘤侵袭、转移中的作用。方法收集2018年5月至2019年6月就诊于广西壮族自治区人民医院临床肿瘤中心的NSCLC患者共62例。采用Canpatrol^TM CTC技术检测患者外周血中的CTCs及CTCs上CCR9表达水平,对CTCs及其CCR9表达水平与NSCLC患者临床、病理的关系进行分析。结果62例NSCLC患者中56例(90.3%)检测出CTCs。CTCs计数与NSCLC的TNM分期、淋巴结转移、远处转移有关(P<0.05)。在CTCs亚型与NSCLC临床相关性分析中,上皮型CTCs 计数与NSCLC 的TNM 分期(r=0.296,P<0.05)及肿瘤远处转移相关(r=0.273,P<0.05);混合型CTCs 计数与NSCLC 的肿瘤转移也存在相关性(r=0.253,P=0.047);且混合型CTCs上CCR9阳性率亦与NSCLC 远处转移相关(r=0.353,P=0.038)。结论CTCs计数及分型与NSCLC的TNM分期及转移相关。CTCs上CCR9表达水平有望用作评估NSCLC肿瘤转移风险的生物标志物。Objective Circulating tumor cells (CTCs) have potential value in the clinical application of various tumors. This study was to investigate the role of CTCs and their chemokine receptor CCR9 in the invasion and metastasis of non-small cell lung cancer (NSCLC). Methods From May 2018 to June 2019,a total of 62 patients with NSCLC in the clinical oncology center of The People's Hospital of Guangxi Zhuang Autonomous Region were enrolled in this study. The CanpatrolTM CTC technique was used to detected the expressions of CTCs and CCR9 in CTCs in peripheral blood of patients. Furthermore,the relationships between expression levels of CTCs,CCR9 and clinical,pathological characteristics of NSCLC patients were analyzed. Results CTCs were detected in 56 of 62(90.3%)NSCLC patients. CTCs counts were associated with TNM stage,lymph node metastasis and distant metastasis of NSCLC(P<0.05). In the analysis of clinical correlation between CTC subtypes and NSCLC,epithelial CTCs counts were related to TNM stage and distant metastasis of NSCLC(r=0.296 and r=0.273,P<0.05). Additionally,counts of mixed type CTCs were also correlative with NSCLC tumor metastasis(r =0.253,P =0.047). Finally,we found that the positive rate of CCR9 in mixed type CTCs was associated with distant metastasis of NSCLC(r=0.353,P=0.038). Conclusion CTCs counts and subtypes were correlated with TNM stage and metastasis of NSCLC. The expression level of CCR9 on CTC was expected to be a biomarker to evaluate the risk of tumor metastasis in NSCLC.

关 键 词：循环肿瘤细胞 非小细胞肺癌 趋化因子受体9 相关性 

分 类 号：R734.2[医药卫生—肿瘤]