下调微RNA-191靶向促进PLCD1表达抑制舌鳞癌细胞侵袭和迁移  

Down-regulation of miR-191 targeted PLCD1 expression on inhibition of invasion and migration of tongue squamous cell carcinoma cells

作　　者：蔡敏[1] 武云霞[2] 王翔宇[3] Cai Min;Wu Yunxia;Wang Xiangyu(Department of Stomatology, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, Shanxi 046000, China;Department of Stomatology, First Clinical Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi 030001, China;Department of Endodontics, Stomatological Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China)

机构地区：[1]长治医学院附属和平医院口腔科,山西长治046000 [2]山西医科大学附属第一临床医院口腔科,太原030001 [3]山西医科大学口腔医院牙体牙髓科,太原030001

出　　处：《中华生物医学工程杂志》2019年第3期313-317,共5页Chinese Journal of Biomedical Engineering

基　　金：山西省教育厅项目(2017JD21).

摘　　要：目的研究微RNA(miR)-191对舌鳞癌细胞侵袭和迁移能力的影响。方法Realtime PCR方法测定舌鳞癌Cal-27、CTST-1、SCC9细胞和正常口腔上皮HOK细胞中miR-191表达变化。在舌鳞癌细胞中转染miR-191 inhibitors,MTT方法测定细胞增殖变化,Transwell小室测定细胞侵袭和迁移。预测转染磷脂酶Cδ1(PLCD1)可能是miR-191的靶基因,荧光素酶报告系统鉴定靶向关系。在舌鳞癌细胞中共转染miR-191 inhibitors和PLCD1 siRNA,检测细胞增殖、侵袭和迁移能力变化。结果舌鳞癌Cal-27、CTST-1、SCC9细胞中miR-191水平高于正常口腔上皮HOK细胞。转染miR-191 inhibitors后的舌鳞癌细胞中miR-191表达水平降低,细胞增殖、侵袭和迁移能力降低。miR-191靶向调控PLCD1。共转染miR-191 inhibitors和PLCD1 siRNA后的舌鳞癌细胞中PLCD1蛋白水平有所降低,细胞增殖、侵袭和迁移能力有所升高。结论下调miR-191靶向促进PLCD1表达抑制舌鳞癌细胞侵袭和迁移。Objective To determine the effect of microRNA (miR)-191 on the invasion and migration of tongue squamous cell carcinoma cells. Methods Realtime PCR was used to examine the expression of miR-191 in tongue squamous cell carcinoma cell lines Cal-27, CTST-1 and SCC9 and normal oral epithelial HOK cells. miR-191 inhibitors were transfected into tongue squamous cell carcinoma cells. The cell proliferation was measured by MTT assay, and the cell invasion and migration were measured by Transwell chamber. It was predicted that the transfected phospholipase Cδ1 (PLCD1) may be the target gene of miR-191. Luciferase reporting system was used to identify the targeting relationship. miR-191 inhibitors and PLCD1 siRNA were co-transfected into tongue squamous cell carcinoma cells to determine cell proliferation, invasion and migration. Results The level of miR-191 in tongue squamous cell carcinoma cell lines Cal-27, CTST-1 and SCC9 was higher than that in normal oral epithelial HOK cells. The expression level of miR-191 in tongue squamous cell carcinoma cells transfected with miR-191 inhibitors decreased, and the cell proliferation, invasion and migration ability decreased. miR-191 targeted and regulated PLCD1. The level of PLCD1 protein in tongue squamous cell carcinoma cells co-transfected with miR-191 inhibitors and PLCD1 siRNA decreased, whereas the cell proliferation, invasion and migration increased. Conclusion Down-regulation of miR-191 targeted PLCD1 expression may inhibit invasion and migration of tongue squamous cell carcinoma cells.

关 键 词：舌鳞癌 微RNA-191 PLCD1 侵袭 

分 类 号：R739.86[医药卫生—肿瘤]