循环肿瘤细胞与早期乳腺癌临床病理特征的相关性  

Correlation between circulating tumor cells and clinicopathological features of early breast cancer

作　　者：龚嘉 徐峰[1] 周美容 吴玉芳 谢萍芳[1] GONG Jia;XU Feng;ZHOU Meirong;WU Yufang;XIE Pingfang(Department of Breast Surgery,Second Xiangya Hospital,Central South University,Changsha 410011,China)

机构地区：[1]中南大学湘雅二医院乳腺专科

出　　处：《中南大学学报（医学版）》2019年第9期1016-1022,共7页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(2019JJ40452);湖南省发改委资助课题(湘财企指[2016]65号)~~

摘　　要：目的:探讨外周血循环肿瘤细胞(circulating tumor cells,CTCs)数量及分子表型与早期乳腺癌临床病理特征的相关性。方法:收集中南大学湘雅二医院乳腺外科某个乳腺癌治疗小组2017年1月至2018年12月收治的100例早期乳腺癌患者的临床病理资料,采用Canpatrol^TM CTCs检测技术检测患者外周血中的CTCs,并予以计数和分子表型鉴定。结果:在早期乳腺癌患者外周血中检测到CTCs的阳性率为90%,分子表型以混合型CTCs居多(55.6%);CTCs阳性仅与肿瘤病理类型相关(P<0.05),与其他临床病理特征无关;CTCs总数、上皮型CTCs数量、混合型CTCs数量均与临床病理特征无相关性,但间质型CTCs数量与激素受体(本文特指雌激素受体和孕激素受体)的表达及Ki-67的表达相关(均r=0.200,P<0.05),间质型CTCs比例与Ki-67的表达状态相关(r=0.213,P<0.05)。结论:CTCs数量与早期乳腺癌患者的临床病理特征均不相关,但在激素受体阴性、Ki-67高表达的早期乳腺癌患者中可能出现更多的间质型CTCs。Objective:To investigate the correlation between the number of peripheral blood circulating tumor cells (CTCs) and clinicopathological features of early breast cancer. Methods:The clinical and pathological data from 100 patients with early breast cancer treated by a breast cancer treatment team in the Department of Breast Surgery,Second Xiangya Hospital, Central South University,were collected from January 2017 to December 2018.For these patients, their peripheral blood CTCs were detected,enumerated and typed by CanpatrolTM CTC assay. Results:The positive rate of CTCs was 90% in peripheral blood of patients with early breast cancer, and the majority of molecular phenotypes was hybrid CTCs (55.6%).The positive rate of CTCs was only related to the pathological type of tumor (P<0.05),but not to other clinicopathological features.No correlation between clinicopathological features and the total number of CTCs, the number of epithelial CTCs or the number of hybrid CTCs was found.However,the number of mesenchymal CTCs was significantly correlated with the expression of hormone receptors and Ki-67 (r=0.200,P<0.05),and there was a significant correlation between the proportion of mesenchymal CTCs and the expression level of Ki-67 (r=0.213,P<0.05). Conclusion:The number of CTCs is not correlated with all clinicopathological features,but patients with negative hormone receptor and high expression of Ki-67 probably have more hybrid CTCs.

关 键 词：乳腺癌 循环肿瘤细胞 表型 临床病理特征 

分 类 号：R737.9[医药卫生—肿瘤]