正畸应力作用下牙龈肌成纤维细胞表达的初步研究  

Expression of myofibroblast in gingival after orthodontic loading

作　　者：孟耀[1] 刘曼 邓倩楠 Meng Yao;Liu Man;Deng Qiannan(Dept. of Orthodontics,Shenzhen Children’s Hospital,Shenzhen 518000,China;Medical Technic & Nursing School,Shenzhen Polytechnic,Shenzhen 518055,China)

机构地区：[1]深圳市儿童医院口腔正畸科,深圳518000 [2]深圳市职业技术学院医学技术及护理学院,深圳518055

出　　处：《华西口腔医学杂志》2019年第5期537-540,共4页West China Journal of Stomatology

基　　金：深圳市科技创新委员会基础研究项目(JCYJ20180228164957104)~~

摘　　要：目的初步探讨正畸应力加载后牙龈肌成纤维细胞的表达情况。方法选取8例正畸应力加载后需要拔除牙牙龈组织,以应力加载前拔除牙牙龈组织为对照,进行α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原、Ⅲ型胶原免疫组织化学染色。然后进行测量和统计分析。结果正畸应力加载前牙龈组织内Ⅰ型胶原、Ⅲ型胶原免疫组化染色阳性,α-SMA除血管上皮外,其余为阴性表达。正畸应力加载后,Ⅰ型胶原、Ⅲ型胶原表达明显上升,差异有统计学意义(P<0.05);α-SMA在牙龈组织内出现阳性表达,与加力前差异有统计学意义(P<0.05)。结论正畸应力加载后,牙龈组织内肌成纤维细胞出现表达,其可能在正畸牙术后复发中发挥一定作用。Objective To examine the expression of myofibroblast in gingival after orthodontic loading. Methods Eight patients were selected as experimental group and treated with orthodontic force for 4 months. Ten patients were selected as the control group, were not loaded. The gingival protein expressions of collagen typeⅠ, collagen type Ⅲ,α-smooth muscle actin (α-SMA) were evaluated by immunohistochemistry method. Results Positive expressions of collagen typeⅠ, collagen type Ⅲ were founded, while no positive staining for α-SMA in the gingival tissue except vascular epithelium before loading. In experimental group, collagen type I and collagen type Ⅲ were increased after orthodontic loading (P<0.05), the expression of α-SMA was detected and statistically significant (P<0.05). Conclusion The myofibroblast exists in gingival tissue after orthodontic loading, and it may be concerned with orthodontic teeth relapse.

关 键 词：正畸 牙龈 肌成纤维细胞 Α-平滑肌肌动蛋白 

分 类 号：R783.5[医药卫生—口腔医学]