肾上腺髓质素在人骨肉瘤组织中的表达  

Expression of adrenomedullin in human osteosarcoma

作　　者：戴星[1] 杨康平[1] 尹战海[1] 马巍[1] 杨益民[1] 张党锋[1] 周晓玲[1] 张匡华 姚璐 DAI Xing;YANG Kangping;YIN Zhanhai;MA Wei;YANG Yimin;ZHANG Dangfeng;ZHOU Xiaoling;ZHANG Kuanghua;YAO Lu(Deptartment of Orthopaedics,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China;Deptartment of Physiology and Pathophysiology,School of Basic Medical Sciences of Xi’an Jiaotong University Health Science Center)

机构地区：[1]西安交通大学第一附属医院骨科,西安710061 [2]西安交通大学医学部基础医学院生理学与病理生理学系

出　　处：《山西医科大学学报》2019年第9期1218-1223,共6页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81702669,31300899);陕西省重点研发计划资助项目(2017SF-231);陕西省自然科学基金资助项目(2018JM7035);中央高校基本科研业务费项目(XJJ2018046);陕西省大学生创新创业训练计划项目;西安交通大学医学部本科开放实验项目

摘　　要：目的 检测肾上腺髓质素(adrenomedullin,ADM)在不同组织和手术前后的差异化表达。方法 通过免疫组化和RT-PCR检测20例骨肉瘤组织和瘤旁组织,10例正常组织中ADM表达情况;放射免疫法检测20例骨肉瘤患者术前、术后2周及10例正常人血浆中ADM的含量。结果 ADM在人骨肉瘤组织呈强阳性表达,骨肉瘤组织中的ADM mRNA表达量明显高于瘤旁组织和正常组织( P <0.05)。骨肉瘤患者术前血浆ADM含量高于健康人血浆ADM含量( P <0.05);骨肉瘤患者术后血浆ADM含量低于术前血浆ADM含量( P <0.05)。结论 ADM在人骨肉瘤组织中呈高表达,肿瘤经手术切除后ADM表达量随之降低,ADM有可能作为基因治疗新的作用靶点。Objective To investigate the differential expression of adrenomedullin(ADM) in osteosarcoma, paratumoral tissues and normal tissues, before and after surgical operation. Methods The expression of ADM in 20 cases of osteosarcoma tissues, 20 cases of paratumoral tissues and 10 cases of normal tissues was detected by immunohistochemistry and RT-PCR. The plasma level of ADM was detected by radioimmunoassay in 20 osteosarcoma patients and 10 normal controls before operation and two weeks after operation. Results ADM was strongly positive in human osteosarcoma, and the level of ADM mRNA in osteosarcoma tissues was significantly higher than that in paratumoral tissues and normal tissues( P <0.05). The plasma ADM level of osteosarcoma patients before operation was higher than that of healthy controls( P <0.05). ADM level in osteosarcoma patients was decreased after operation( P <0.05). Conclusion ADM is highly expressed in osteosarcoma tissues, and ADM expression is decreased after tumor resection. ADM may become a new target for gene therapy.

关 键 词：骨肉瘤 肾上腺髓质 降钙素 

分 类 号：R738.1[医药卫生—肿瘤]