全自动免疫组化法与手工免疫组化法检测卵巢癌ALK蛋白表达的对比分析  

Comparative Study of ALK Antibody in Ovarian Cancer between Manual and Automatic Immunohistochemical Detection

作　　者：罗文姬[1] 李瑞珍[1] 罗清平[1] 余光银[2] 魏蔚霞[1] LUO Wen-ji;YU Guang-yin;LI Rui-zhen(Department of Gynaecology and Obstetrics,Peking University Shenzhen Hospital,Shenzhen 518036,Guangdong Province,China)

机构地区：[1]北京大学深圳医院妇产科,广东深圳518036 [2]北京大学深圳医院病理科,广东深圳518036

出　　处：《罕少疾病杂志》2019年第5期35-37,共3页Journal of Rare and Uncommon Diseases

摘　　要：目的探讨全自动免疫组化法与手工免疫组化法检测ALK(anaplastic lymphoma kinase,ALK)在卵巢癌中的表达,并对两者进行比较。方法选取53例卵巢癌组织的石蜡病理,应用抗体ALK(D5F3)全自动IHC法联合抗ALK(5A4)手工IHC法分别检测卵巢癌中ALK蛋白水平,并对两种方法进行比较。结果抗ALK(D5F3)检测发现ALK在卵巢癌组织中表达,阳性率9.43%(5/53),而抗ALK(5A4)组化法未检测出卵巢癌组织中有ALK蛋白表达。结论ALK蛋白在卵巢癌中有表达,抗D5F3全自动IHC法优于抗5A4手工免疫组化法,抗D5F3联合全自动免疫组化法敏感度更高。Objective To investigate the detection and comparison of ALK(ALK)in ovarian cancer by automatic immunohistochemistry and manual immunohisto-chemistry.Methods The paraffin pathology of 53 ovarian cancer tissues was selected,and ALK protein levels in ovarian cancer were detected by ALK(D5F3)automatic IHC method combined with ALK(5A4)manual IHC method,and the two methods were compared.Results Anti-alk(D5F3)test showed that ALK was expressed in ovarian cancer tissues with a positive rate of 9.43%(5/53),while anti-alk(5A4)histochemistry method did not detect the expression of ALK protein in ovarian cancer tissues.Conclusion ALK protein is expressed in ovarian cancer.The automatic IHC method against D5F3 is superior to the manual immunohistochemical method against 5A4.

关 键 词：卵巢癌 间变性淋巴瘤激酶(ALK) 全自动免疫组化学(IHC) 

分 类 号：R737.31[医药卫生—肿瘤]