microRNA在皮肤黑色素瘤血清与毛发中的差异表达  

Differential expression of microRNAs in serum and hairs different position of cutaneous melanoma

作　　者：林千里 张文俊 王中志 汪汇 江华 LIN Qianli;ZHANG Wenjun;WANG Zhongzhi;WANG Hui;JIANG Hua(Department of Plastic Surgery,Shanghai Changzheng Hospital,Navy Military Medical University,Shanghai 200003,China)

机构地区：[1]海军军医大学附属长征医院整形外科

出　　处：《中国医药导报》2019年第26期7-11,16,共6页China Medical Herald

基　　金：国家青年科学基金资助项目（81601212）

摘　　要：目的探讨皮肤恶性黑色素瘤miRNA的差异表达有望为该肿瘤的诊断和靶向治疗提供依据,筛选皮肤恶性黑色素瘤血清和毛发2个部位的7个特异性miRNA表达差异。方法收集2016年3月~2017年10月在海军军医大学附属长征医院手术局部切除并经常规组织病理学和免疫组织化学检测证实为恶性皮肤黑色素瘤(CM)的12个病例作为CM组,同期收集非肿瘤疾病的17个皮肤病病例作为对照组。采用qRT-PCR技术检测两组患者的血清和毛发中miRNA表达情况。将检测结果一致的候选miRNA确定为有意义的共同差异表达生物学指标,利用组间差异倍数筛选出差异>2.5倍差异表达的miRNA。结果 CM组患者血清中7个miRNA有2个上调,5个下调,上调的miRNA包括miR-4487和miR-4706,下调的miRNA包括miR-16、miR-211、miR-4731、miR-509-3p和miR-514a;毛发中有3个上调,4个下调,上调的miRNA包括miR-211、miR-4487和miR-4731;下调的miRNA包括miR-16、miR-4706、miR-509-3p和miR-514a。与对照组比较,CM组miR-4487在血清和毛发中共同上调,但差异无统计学意义(P> 0.05)。与对照组比较,CM组血清和毛发中has-miR-16和has-miR-509-3p表达显著下调,差异均有统计学意义(P <0.05),并且一致性较高;与对照组比较,CM组血清和毛发中miR-541a表达虽然显示共同下调,但差异无统计学意义(P> 0.05)。血清和毛发差异表达2.5倍以上的miRNA为miR-16和miR-514a;单独毛发差异表达2.5倍以上的miRNA为miR-4706;单独血清标本4倍以上差异表达为miR-4731;血清标本10倍以上差异表达的miRNA为miR-211和miR-509-3p。结论与非肿瘤疾病患者比较,血清和毛发中存在多种miRNA的差异表达,CM患者血清中miR-211和miR-509-3p存在高度显著差异表达,可以作为该肿瘤的生物学标志物;毛发标本miR-4706的差异表达有可能成为黑色素瘤早期非创伤性检测的生物学指标。miRNA检测对CM是可利用的生物学指标,也可能成为今后靶基因治疗的�Objective To investigate the differential expression of microRNAs in cutaneous malignance melanoma in order to provide a basis for the diagnosis and targeted treatment of this malignancy;to screen seven specific microRNAs in serum and hairs of cutaneous malignancy melanoma. Methods From March 2016 to October 2017, 12 cases of cuta- neous melanoma (CM) confirmed by routine histopathology and immunohistochemistry in Shanghai Changzheng Hospi- tal, Navy Military Medical University, were collected as CM group, while 17 cases of non-neoplastic skin diseases were collected as control group. The expression situation of microRNAs in serum and hair of two groups were detected by qRT-PCR. Candidate microRNAs with consistent results were identified as significant common differential expression biological indicators. The differentially expressed microRNAs (> 2.5 times) were screened by the multiple of differences between groups. Results There were 2 up-regulated and 5 down-regulated microRNAs in serum among 7 microRNAs of patients in CM group. The up-regulated microRNAs included miR-4487 and miR-4706, the down-regulated microRNAs included miR-16, miR-211, miR-4731, miR-509-3p and miR-514a. There were 3 up-regulated and 4 down-regulated microRNAs in hairs, the up-regulated microRNAs included miR-211, miR-4487 and miR- 4731;the down-regulated microRNAs included miR- 16, miR-4706, miR-509-3p and miR-514a. The coup- regulated microRNAs in serum and hairs of the CM group were microRNAs-4487, but there was no statistically significant difference between the two groups (P > 0.05). Compared with the control group, the expressions of has-microRNA-16 and has-microRNA-509-3p were significantly down-regulated, the differences were statistically significant (P < 0.05), and with high consistency. Compared with control group, the expression of miRNA-541a in serum and hairs of CM group was down-regulated, but there was no sta- tistically significant difference (P > 0.05). MiRNAs with more than 2.5 times differential expression in serum and

关 键 词：皮肤黑色素瘤 微小RNA 信号转导通路 差异表达 

分 类 号：R739.5[医药卫生—肿瘤]