EB病毒基因片段表达水平与鼻咽癌发病的关系  

Relationship between the expression level of EB virus gene fragment and the pathogenesis of nasopharyngeal carcinoma

作　　者：刘洁琼 曹永清 李科 齐慧 LIU Jieqiong;CAO Yongqing;LI Ke;QI Hui(Department of Hematology and Oncology,the First Hospital of Changsha,Hu'nan Province,Changsha 410005,China)

机构地区：[1]长沙市第一医院血液肿瘤科

出　　处：《中国医药导报》2019年第26期113-116,共4页China Medical Herald

基　　金：湖南省卫生计生委科研计划课题项目（B2017222）

摘　　要：目的探讨BCRF-1 mRNA表达水平与鼻咽癌发病相关性及其对免疫功能的影响。方法选取2017年1月~2018年5月于长沙市第一医院(以下简称“我院”)就诊的54例鼻咽癌患者(鼻咽癌组)作为研究对象,选取我院同期就诊的50例非肿瘤性鼻咽患者作为对照组。采用实时荧光定量PCR检测BCRF-1 mRNA的表达水平,采用流式细胞术检测T淋巴细胞亚群。结果与对照组比较,鼻咽癌组BCRF-1 m RNA相对表达显著升高(P <0.05);TNM分期T3~T4期、N2~N3期鼻咽癌BCRF-1 mRNA相对表达量明显高于T1~T2期、N0~N1期(P <0.05);BCRF-1 mRNA高表达组CD4+T细胞比例和CD4+/CD8+明显低于BCRF-1 mRNA低表达组,而CD8+T细胞比例明显高于BCRF-1 mRNA低表达组(均P <0.05)。结论 BCRF-1 m RNA表达可能与鼻咽癌TNM分期T、N有关,同时其高表达对患者免疫功能有抑制作用。Objective To explore the correlation of BCRF-1 mRNA expression with nasopharyngeal carcinoma and its influence on immune function. Methods A total of 54 patients with nasopharyngeal carcinoma (nasopharyngeal carcinoma group) who were admitted to the First Hospital of Changsha (“our hospital” for short) from January 2017 to May 2018 were enrolled in the study. At the same time, 50 cases of non-neoplastic nasopharyngeal patients treated at the same time in our hospital were selected as the control group. The expression of BCRF-1 mRNA was detected by real-time PCR, the T cell subsets were determined by flow cytometry. Results Compared with control group, the relative expression of BCRF-1 mRNA was significantly increased (P < 0.05). And the relative expression of BCRF-1 mRNA in TNM stage T3-T4 and N2-N3 was significantly higher than that in TNM stage T1-T2 and N0-N1 stage (P < 0.05). The percentage of CD4+ T cells and CD4+/CD8+ in the high expression of BCRF-1 mRNA group was significantly lower than that in the low expression of BCRF-1 mRNA group, while the proportion of CD8+ T cells was significantly higher than that in the low expression of BCRF-1 mRNA group (all P < 0.05). Conclusion The expression of BCRF-1 mRNA are may be related to TNM stage T and N of nasopharyngeal carcinoma, and its high expression can inhibit the immune function of patients.

关 键 词：BCRF-1 mRNA 鼻咽癌 EB病毒 免疫功能 临床病理特征 

分 类 号：R730.231[医药卫生—肿瘤]