唐古特白刺果实花色苷改善高脂饮食诱导的小鼠非酒精性脂肪肝损伤  

Anthocyanins from the Fruits of Nitraria tangutorun Bobr. Improve Nonalcoholic Fatty Liver Injury Induced by High-Fat Diet in Mice

作　　者：李冰[1] 刘康 张锦 李翠芹[1,2] LI Bing;LIU Kang;ZHANG Jin;LI Cuiqin(National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest China,Key Laboratory of Pharmaceutical Plant Resources and Natural Pharmaceutical Chemistry,Ministry of Education,Shaanxi Normal University,Xi’an 710119,China;College of Life Sciences,Shaanxi Normal University,Xi’an 710119,China)

机构地区：[1]陕西师范大学西北濒危药材资源开发国家工程实验室,药用植物资源与天然药物化学教育部重点实验室,陕西西安710119 [2]陕西师范大学生命科学学院,陕西西安710119

出　　处：《食品科学》2019年第19期217-223,共7页Food Science

基　　金：中央高校基本科研业务费专项资金项目(GK201603110;GK201706009)

摘　　要：目的:研究唐古特白刺果实花色苷对高脂饮食诱导的C57BL/6J小鼠非酒精性脂肪肝损伤的保护作用及其机制。方法:将C57BL/6J小鼠随机分为对照组、模型组及唐古特白刺果实花色苷组,持续喂养14周。分别测定各组小鼠体质量、腹部脂肪质量、脂体指数、内脏脂肪质量、体长和Lee’s指数等常规生理指标,谷草转氨酶(aspartate aminotransferase,AST)和谷丙转氨酶(alanine aminotransferase,ALT)活力、总胆固醇(total cholesterol,TC)和总甘油三酯(totaltriglyceride,TG)质量浓度、低密度脂蛋白(lowdensitylipoprotein,LDL)和高密度脂蛋白(highdensitylipoprotein,HDL)浓度等生化指标,丙二醛(malondialdehyde,MDA)含量、超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力等氧化应激相关指标,以及乙酰辅酶A羧化酶(acetyl coenzyme A carboxylase,ACC)、肝X受体α(liver X receptor-alpha,LXR-α)、脂肪酸转位酶(fatty acid translocase,CD36)和过氧化物酶增殖体激活受体-α(peroxisome proliferator-activatedreceptor-alpha,PPAR-α)等肝脏脂质代谢相关蛋白质量浓度。结果:与模型组比较,唐古特白刺果实花色苷能明显降低高脂小鼠的体质量、腹部脂肪质量、脂体指数、内脏脂肪质量以及Lee’s指数,降低高脂小鼠血清中AST和ALT活力、TC和TG质量浓度、LDL浓度,提高HDL浓度,降低肝组织中MDA含量和SOD活力,提高GSH-Px活力,降低肝组织脂肪合成相关蛋白ACC、LXR-α和CD36质量浓度,升高脂肪分解相关蛋白PPAR-α质量浓度。结论:唐古特白刺果实花色苷通过调节肝脏氧化应激和脂质代谢,改善高脂饮食诱导的小鼠非酒精性脂肪肝损伤。Objective: To investigate the effect of anthocyanins from Nitraria tangutorun Bobr. fruits on nonalcoholic fatty liver injury induced by high-fat diet(HFD) in C57 BL/6 J mice and its underlying mechanism. Methods: Male C57 BL/6 J mice were randomly divided into control, model and anthocyanin treatment groups. The feeding period lasted for 14 weeks. Routine physiological parameters(body mass, abdominal fat mass, body fat index, visceral fat mass, body length and Lee’s index) were measured in each mouse. Serum samples were collected to detect the activities of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) and the concentrations of total cholesterol(TC), total triglyceride(TG), low density lipoprotein(LDL) and high density lipoprotein(HDL). Besides, liver samples were collected to detect oxidative stress-related indicators such as malondialdehyde(MDA), superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px), and the expression of liver lipid metabolism-related proteins such as acetyl coenzyme A carboxylase(ACC), liver X receptor-alpha(LXR-α), fatty acid translocase(CD36) and peroxisome proliferator-activated receptor-alpha(PPAR-α). Results: Compared with the high-fat model group, the anthocyanins from N. tangutorun Bobr. significantly reduced body mass, abdominal fat mass, body fat index, visceral fat mass and Lee’s index. The levels of serum AST, ALT, TC, TG and LDL were significantly reduced and HDL level in high-fat diet-fed mice was significantly increased after administration with the anthocyanins. In addition, the anthocyanins significantly decreased MDA content and SOD activity and increased GSH-Px activity, as well as significantly downregulated the expression of ACC, LXR-α and CD36 and upregulated the expression of PPAR-α in liver tissue. Conclusion: The anthocyanins from N. tangutorun Bobr. can ameliorate HFD-induced NAFLD in mice by regulating oxidative stress and lipid metabolism in hepatocytes.

关 键 词：唐古特白刺果实花色苷 非酒精性脂肪肝损伤 氧化应激 脂质代谢 

分 类 号：R285.5[医药卫生—中药学] TS218[医药卫生—中医学]