6对通用引物检测不同型别人乳头瘤病毒效果分析  

Detection efficacy of 6 universal primers in different types of papillomavirus

作　　者：吴瑶 吴亮[2] 阴晴[1] 周亚玲 何蕾 戴晓玥 夏雯 许化溪[2] WU Yao;WU Liang;YIN Qing;ZHOU Ya-ling;HE Lei;DAI Xiao-yue;XIA Wen;XU Hua-xi(Department of Clinical Laboratory,Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001;School of Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China)

机构地区：[1]江苏大学附属医院检验科,江苏镇江212001 [2]江苏大学医学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2019年第5期405-409,共5页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省2018年度预防医学科研课题(Y2018108);镇江市社会发展项目(SH2017024)

摘　　要：目的:比较6对通用引物检测9种不同型别人乳头瘤病毒(HPV)的效果,为临床提供一种快速简便的HPV检测方法。方法:收集女性宫颈脱落细胞标本954例,使用HPV分型试剂盒进行HPV-DNA检测并分型。分别使用6对通用引物(①GP5/GP6,②GP5+/GP6+,③CP-Ⅰ/CP-ⅡS,④CP-I/CP-ⅡG,⑤外:MY09/MY11,内:GP5+/GP6+,⑥SPF1/GP6++)对HPV-DNA阳性标本进行PCR检测,比较6对通用引物检测9种不同类型HPV-DNA效果。结果:采用HPV分型试剂盒从954例标本中共检出263例HPV-DNA阳性标本,阳性率为27.57%。在阳性标本中,采用通用引物SPF1/GP6++检出的阳性例数为223例,检出率为84.79%;采用巢氏PCR引物(外:MY09/MY11,内:GP5+/GP6+)检出的阳性例数为207例,检出率为78.71%。上述两对通用引物均可以检出9种型别HPV-DNA。结论:通用引物SPF1/GP6++检测效果最佳,但引物含次黄嘌呤,成本较高;巢氏PCR引物(外:MY09/MY11,内:GP5+/GP6+)检测效果次之,且合成成本较低。在HPV研究中可以根据实际情况选用。Objective:To compare the detection efficacy of 6 universal primers in detecting 9 different types of human papillomavirus(HPV)and provide a quick and easy screening method for clinical studies.Methods:We collected 954 female cervical exfoliated cell samples,then detected and classified the HPV-DNA with HPV typing kit.Then we used 6 pairs of universal primers(①GP5/GP6,②GP5+/GP6+,③CP-Ⅰ/CP-ⅡS,④CP-I/CP-ⅡG,⑤external:MY09/MY11,internal:GP5+/GP6+,⑥SPF1/GP6++)to detect HPV-DNA positive samples by PCR and compared their detection effect in 9 types of HPV-DNA.Results:A total of 263 HPV-DNA positive samples were detected from 954 specimens with HPV typing kit,the HPV infection rate was 27.57%.In the HPV-DNA positive samples,the positive cases detected by SPF1/GP6++was 223,and the detection rate was 84.79%.But 207 positive cases were detected by nested PCR primers(external:MY09/MY11,internal:GP5+/GP6+),the corresponding detection rate was 78.71%.The two universal primers above were able to detect all 9 HPV-DNA types.Conclusion:The universal primer SPF1/GP6++has the best detection efficacy,but the synthesis cost is higher because of containing hypoxanthine.The nest PCR primer(external:MY09/MY11,inner:GP5+/GP6+)has the second highest detection efficacy,and the synthesis cost is lower.Therefore,we may select suitable universal primers according to the actual situation in HPV detection.

关 键 词：人乳头瘤病毒 通用引物 HPV基因型 PCR 

分 类 号：R737.33[医药卫生—肿瘤]