Nanopore-based DNA Supersandwich Structure for Detection of Streptavidin  

作　　者：QIAO Yujuan QIAN Yue LIU Mengfei LIU Nnanan TANG Xingxing 

机构地区：[1]Key Laboratory of Carbon Materials of Zhejiang Province,College of Chemistry & Materials Engineering,Wenzhou University,Wenzhou 325027,P.R.China [2]Department of Materials Engineering,Zhejiang Industry & Trade Vocational College,Wenzhou 325003,P.R.China

出　　处：《Chemical Research in Chinese Universities》2019年第5期837-841,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.21505101);the Natural Science Foundation of Zhejiang Province,China(No.LQ16B050003).

摘　　要：Natural and syntlietic nanopores are increasingly popular tools in biosensors. In this work, the DNA supersandwich structure, which was made from two specially designed probes has been used to be fabricated in solid nanopores. Integrating the idea of affinity between streptavidin and biotin, the DNA supersandwich structure with biotins was successfully constructed for streptavidin detection, and the limitation of detection was found to be 10 fmol/L. This nanodevice allows specific, sensitive and versatile detection of diverse analytes with easy operations, thus we believe that it could be developed to detect some disease-related molecular targets and play a considerable role in biotechnology.

关 键 词：DNA supersandwich structure STREPTAVIDIN DETECTION Solid-nanopore 

分 类 号：O6[理学—化学]