小鼠急性酒精性肝损伤过程中热休克蛋白Gp96的表达变化及意义  

Expression and significance of heat shock protein Gp96 during acute alcoholic liver injury in mice

作　　者：宋影 李三强[1] 田张钰 李子昂 代新华 王玉东 苏立鹏 宋晓改 朱文枫 杨欢 SONG Ying;LI Sanqiang;TIAN Zhangyu;LI Ziang;DAI Xinhua;WANG Yudong;SU Lipeng;SONG Xiaogai;ZHU Wenfeng;YANG Huan(The Molecular Medicine Key Laboratory of Liver Injury and Repair, Medical College, Henan University of Science and Technology, Luoyang 471003, China)

机构地区：[1]河南科技大学医学院肝损伤与修复分子医学重点实验室

出　　处：《胃肠病学和肝病学杂志》2019年第10期1148-1151,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然基金资助项目(81201558);河南省科技创新杰出青年(184100510006);河南省高校科技创新团队项目(181RTSTHN026);河南科技大学创新团队资助项目(2015XTD003);河南省科技攻关项(172102310693)

摘　　要：目的研究小鼠急性酒精性肝损伤过程中热休克蛋白Gp96的表达变化及意义。方法选取健康昆明雄性小鼠50只随机分为2组:正常组(n=10)、实验组(n=40)。正常组小鼠不做处理,实验组小鼠酒精灌胃(红星二锅头,16ml/kg)诱导小鼠急性肝损伤,分别于灌胃后6、12、18和24h将各组小鼠眼球取血并分离血清,检测谷丙转氨酶(ALT)活性;颈椎脱臼处死小鼠分离提取肝脏,石蜡切片苏木精-伊红染色(HE染色)观察各组小鼠肝组织病理学改变,蛋白免疫印迹法检测小鼠肝组织中热休克蛋白Gp96的表达变化。结果ALT检测结果显示,酒精灌胃后,随着时间增加,血清ALT酶的活性持续升高,18h后达最高值(P<0.01),随着肝损伤的修复,血清ALT酶的活性开始出现下降;HE染色结果显示,酒精灌胃后,随着时间的增加,肝组织出现了不同程度的损伤,18h后损伤积分达到最高值(P<0.01),随着肝组织修复损伤积分有所降低:免疫印迹结果显示,酒精灌胃后Gp96的蛋白表达量随着时间的增加持续升高,18h后达最高值(P<0.01),随着肝损伤的修复,Gp96蛋白表达量开始下降。结论小鼠急性酒精性肝损伤过程中Gp96的变化显著表现为先升高后降低,Gp96可能在肝损伤后的修复中起重要作用。Objective To investigate the expression and significance of heat shock protein Gp96 during acute alcoholic liver injury in mice. Methods Fifty healthy Kunming male mice were randomly divided into 2 groups, normal group ( n =10) and experimental group ( n =40). The mice in the normal group were not treated, the mice in the experimental group were induced by alcoholic gavage (Red Star Erguotou, 16 ml/kg) to induce acute liver injury in mice, blood samples were taken from the eyeballs of each group at 6, 12, 18 and 24 hours after gavage, and serum was separated to detect alanine aminotransferase (ALT) activity. The mice were sacrificed by cervical dislocation and the liver was isolated, paraffin sections were stained with hematoxylin-eosin (HE staining) to observe the pathological changes of liver tissue in each group, the expression of heat shock protein Gp96 was detected by immunoblotting. Results The results of alanine aminotransferase activity (ALT) showed that after alcohol administration, the activity of serum ALT enzyme was increased continuously with time, reached the highest value after 18 hours ( P <0.01), and then with the repair of liver damage, the activity of serum ALT enzyme began to decline;the results of HE staining showed that after alcohol administration, the liver tissue showed different degrees of damage with time, and the injury score reached the highest value after 18 hours ( P <0.01), and then with the repair of liver tissue, the damage score was decreased;the results of immunoblotting showed that the expression of Gp96 protein was increased with time after alcohol administration, and reached the highest value after 18 hours ( P <0.01). Then, with the repair of liver injury, the expression of Gp96 protein began to decrease. Conclusion The expression of Gp96 protein in mice with acute alcoholic liver injury changed significantly, which was firstly elevated and then decreased. It is believed that Gp96 may play an important role in the repair of liver injury.

关 键 词：热休克蛋白GP96 急性酒精性肝损伤 表达变化 

分 类 号：R575[医药卫生—消化系统]