糜烂型口腔扁平苔藓差异表达MicroRNAs的筛选  

Screening of the differentially expressed microRNAs in erosive oral lichen planus tissue

作　　者：高胜男 李言君[1] 李建卫[1] 崔彩云 李焕焕 王雯虹 GAO Shengnan;LI Yanjun;LI Jianwei;CUI Caiyun;LI Huanhuan;WANG Wenhong(Department of Oral Medicine, Binzhou Medical University Hospital, 256600, China)

机构地区：[1]滨州医学院附属医院口腔内科

出　　处：《实用口腔医学杂志》2019年第5期696-700,共5页Journal of Practical Stomatology

基　　金：山东省自然科学基金(编号:ZR2016HL23)

摘　　要：目的:用基因芯片筛选糜烂型口腔扁平苔藓(OLP)病损部位黏膜组织差异表达的MicroRNAs并预测其相关靶基因。方法:采集糜烂型OLP病损黏膜组织和正常口腔黏膜组织各3例,提取总RNA后,用Cyanine 3-pCp标记,标准条件下标记好的探针在Agilent微阵列上杂交,Agilent微阵列扫描仪扫描芯片,使用Agilent Feature Extraction软件分析数据,筛选出具有统计学意义的差异表达miRNAs。采用miRNAs生物信息学技术预测差异表达明显的miRNAs的靶基因。结果:糜烂型OLP和正常口腔黏膜组织中提取的总RNA均符合芯片杂交要求,RNA完整性评分1.8~2.0;筛选获得159个与糜烂型OLP相关的miRNAs,其中12个miRNAs表达上调,147个miRNAs表达下调。生物信息学分析得出miR-206的预测靶基因中5个与糜烂型OLP相关。结论:筛选得到的糜烂型OLP病损黏膜中差异表达的miRNAs可能与糜烂型OLP发病相关。Objective: To screen differentially expressed microRNAs in mucosal tissues of erosive oral lichen plauns (OLP) lesions and predict their related target genes. Methods: Total RNA was extracted from 3 cases of erosive OLP lesions and 3 of normal oral mu cosa and labeled with Cyanine 3-pCp. The labeled probes under standard conditions were hybridized on Agilent microarrays. Agilent microarray was scanned by the scanning instrument and analyzed using Agilent Feature Extraction software for the analysis of statistically significant differentially expressed miRNAs. miRNAs bioinformatics techniques were used to predict target genes for differentially ex pressed miRNAs. Results:Total RNA extracted from the samples met the requirements of microarray hybridization, and the RNA in tegrity score was 1.8 ~2.0. 159 miRNAs related to erosive OLP were screened, 12 of which were up-regulated and 147 down-regula ted. Bioinformatics analysis revealed that 5 of the predicted target genes of miR-206 were associated with erosive OLP. Conclusion: The differentially expressed miRNAs in the mucosa of the erosive OLP lesion may be related to the pathogenesis of erosive OLP.

关 键 词：口腔扁平苔藓(OLP) MICRORNAS MIRNA芯片 miR-206 miR-133b 

分 类 号：R781.5[医药卫生—口腔医学]