血清S-100B蛋白和一氧化氮检测对急性脑出血的临床评价价值  

Clinical evaluation value of serum S-100B protein and NO detection in acute cerebral hemorrhage

作　　者：王小蓉[1] 杨友京 王显斌 罗小兰 WANG Xiao-rong;YANG You-jing;WANG Xian-bin;LUO Xiao-lan(Department of Geriatrics,Ya′an People′s Hospital,Sichuan Province,Ya′an 625000,China)

机构地区：[1]四川省雅安市人民医院老年病科

出　　处：《中国当代医药》2019年第28期60-63,74,共5页China Modern Medicine

基　　金：四川省卫生厅科研课题(090552)

摘　　要：目的探讨血清S-100B蛋白和一氧化氮(NO)检测对急性脑出血的临床评价价值,分析其与急性脑出血患者的临床症状、体征及预后之间的关系。方法选取2009年1月~2014年1月我院收治的80例急性脑出血患者作为急性脑出血组,选取同期在我院体检的80例健康体检者作为对照组。利用多田公式根据急性脑出血组患者入院头颅CT片计算脑出血血肿体积,再将其分为小量出血组(25例),中量出血组(32例)及大量出血组(23例)。所有患者按照美国国立卫生研究院脑卒中量表(NIHSS)评估神经功能缺损状况,再将0~15分作为预后良好组(52例),16~42分作为预后恶劣组(28例)。采用酶联免疫吸附测定法(ELISA)和硝酸还原酶法动态检测血清S-100B蛋白和NO含量变化;采用Pearson相关性分析血清S-100B蛋白和NO含量与出血量的相关性。结果脑出血第1天,急性脑出血组的血清S-100B蛋白与NO含量高于对照组,差异有统计学意义(P<0.05);急性脑出血组脑出血第3、7、10天的血清S-100B蛋白与NO含量高于脑出血第1天,差异有统计学意义(P<0.05);急性脑出血组的血清S-100B蛋白含量与NO含量在脑出血第3天高于第7、10天,差异有统计学意义(P<0.05)。小量出血组的第1、3、7、10天的血清S-100B蛋白和NO含量低于中量出血组和大量出血组,中量出血组脑出血第1、3、7、10天的血清S-100B蛋白和NO含量低于大量出血组,差异均有统计学意义(P<0.05)。脑出血第1天,预后恶劣组和预后良好组的血清S-100B蛋白和NO含量比较,差异无统计学意义(P>0.05);预后恶劣组的血清S-100B蛋白和NO含量在脑出血第3、7、10天高于脑出血第1天,差异有统计学意义(P<0.05);预后恶劣组在脑出血第3、7、10天的血清S-100B蛋白和NO含量高于预后良好组,差异有统计学意义(P<0.05)。经Pearson相关性分析发现,血清S-100B蛋白和NO含量与出血量均成正相关(r1=0.76,r2=0.72,P<0.05),且急性脑出血组Objective To explore the clinical value of serum S-100B protein and NO detection in acute cerebral hemorrhage, and to analyze the relationship with clinical symptoms, signs and prognosis of patients with acute cerebral hemorrhage by serum S-100B protein and NO detection. Methods A total of 80 patients with acute cerebral hemorrhage admitted to our hospital from January 2009 to January 2014 were selected as the acute cerebral hemorrhage group, and 80 healthy people who underwent physical examination in our hospital during the same period were selected as the control group. The volume of hematoma of cerebral hemorrhage was calculated by the Doda formula on the basis of head CT films. According to the size of hematoma, 25 patients were divided into small hemorrhage group, 32 patients in medium hemorrhage groupand 23 patients in large hemorrhage group. Neurological deficits were assessed using the national institutes of health stroke inventory (NIHSS). Then 0 to 15 points were used as the group with good prognosis (52 cases), 16 to 42 points as poor prognosis group (28 cases). The concentration of S-100B protein and NO content were detected dynamically by ELISA and nitrate reductase methods, respectively. Results On the first day of cerebral hemorrhage, serum S-100B protein and NO content in the acute cerebral hemorrhage group were higher than that in the control group, and the differences were statistically significant (P<0.05). The serum S-100B protein and NO content on the 3rd, 7th and 10th days of acute cerebral hemorrhage group were higher than that on the 1st day of cerebral hemorrhage, and the difference were statistically significant (P<0.05);The serum S-100B protein content and NO content in the acute cerebral hemorrhage group in the 3 rd day after cerebral hemorrhage was higher than those in the 7th and 10th days after cerebral hemorrhage, and the differences were statistically significant (P<0.05). The serum S-100B protein and NO content on the 3rd, 7th and 10th day in the small amount of bleeding group we

关 键 词：脑出血 血清S-100B蛋白 一氧化氮 脑损伤 临床评价 

分 类 号：R743[医药卫生—神经病学与精神病学]