基于数据库分析人EIF3C在头颈鳞状细胞癌中的表达及临床意义  

Expression and clinical significance of EIF3C in head and neck squamous cell carcinoma utilizing databases

作　　者：赵谦[1] 任婉丽[1] 李宏慧[1] 戴皓 白艳霞[1] 张少强[1] 姚小宝[1] 邵渊[1] Zhao Qian;Ren Wanli;Li Honghui;Dai Hao;Bai Yanxia;Zhang Shaoqiang;Yao Xiaobao;Shao Yuan(Department of Otolaryngology-Head and Neck Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China.)

机构地区：[1]西安交通大学第一附属医院耳鼻喉头颈外科

出　　处：《现代肿瘤医学》2019年第21期3789-3793,共5页Journal of Modern Oncology

基　　金：陕西省科技攻关基金(编号:S2018-JC-YB-1534)

摘　　要：目的:通过研究真核翻译起始因子3C(eukaryotic translation initiation factor 3 subunit C,EIF3C)在头颈鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)组织中的表达,阐明EIF3C基因的表达与HNSCC临床病理特征和预后的关系以及其相互作用蛋白参与的生物学过程。方法:采用GEPIA数据库分析HNSCC和对照组织中EIF3C基因的表达差异以及HNSCC患者中EIF3C基因表达的高低与预后之间的关系。采用LinkedOmics数据库分析EIF3C基因的表达与HNSCC的病理分级以及TNM分期之间的关系。采用The Human Protein Atlas数据库分析EIF3C蛋白在正常和HNSCC组织中的表达水平。采用STRING数据库分析EIF3C相互作用的蛋白以及参与的生物学过程。结果:EIF3C的表达水平在HNSCC中显著增高(P<0.01),与HNSCC的病理分级相关(P=0.026 6),且分别与HNSCC的T分期(P=0.261 8)、N分期(P=0.358 8)和M分期(P=0.3413)不相关。EIF3C基因表达的高低与HNSCC的总生存率(P=0.24,HR=1.2)和无病生存率(P=0.66,HR=0.93)无显著性差异。免疫组化结果显示EIF3C蛋白在正常组织中呈中等水平表达,而在HNSCC组织中呈中、高等水平表达。EIF3C主要与其他EIFs蛋白相互作用,主要参与细胞的翻译起始调控。结论:HNSCC中EIF3C的表达显著增高且与临床病理分级相关,可为后续EIF3C的功能研究提供重要的理论基础。Objective:To elucidate the relationship between expression of eukaryotic translation initiation factor 3 subunit C(EIF3C)and clinical characteristics,prognosis of head and neck squamous cell carcinoma(HNSCC),and the biological process with EIF3C interacting proteins by studying the expression of EIF3C in HNSCC tissues.Methods:The GEPIA database was used to analyze the difference in expression of EIF3C between HNSCC and control tissues,and the relationship between the level of EIF3C gene expression and prognosis in HNSCC patients.The relationship between the expression of EIF3C and the pathological grade of HNSCC and TNM stage was mined by LinkedOmics database.The expression level of EIF3C protein in normal and HNSCC tissues was explored in The Human Protein Atlas database.The STRING database was used to analyze EIF3C interacting proteins and the biological process involved.Results:The expression level of EIF3C was significantly increased in HNSCC(P<0.01),correlated with the pathological grade of HNSCC(P=0.026 6),and T stage(P=0.261 8),N stage(P=0.358 8)and M stage(P=0.341 3)were not relevant.There was no significant difference between EIF3C expression and overall survival(P=0.24,HR=1.2)and disease-free survival(P=0.66,HR=0.93)of HNSCC.Immunohistochemistry results showed that EIF3C protein was expressed at moderate levels in normal tissues and at intermediate and high levels in HNSCC tissues.EIF3C mainly interacted with other EIFs proteins and was mainly involved in the regulation of translational initiation.Conclusion:The expression of EIF3C in HNSCC is significantly increased and correlated with clinical pathological grade,which may provide an important theoretical basis for further study.

关 键 词：真核翻译起始因子3C 头颈鳞状细胞癌 数据库分析 预后 

分 类 号：R739.91[医药卫生—肿瘤]