非小细胞肺癌组织VEGF p-Akt及PTEN的表达及临床意义  

Expression and Clinical Significance of VEGF p-Akt and PTEN in Non-small Cell Lung Cancer

作　　者：张秀义[1] 许浩然[1] 景卫革[1] 何士杰[1] 孙亚东 孙秀娟 ZHANG Xiuyi;XU Haoran;JING Weige(The Central Hospital of Chengde,Hebei Chengde 067000,China)

机构地区：[1]河北省承德市中心医院呼吸内科

出　　处：《河北医学》2019年第10期1598-1603,共6页Hebei Medicine

基　　金：河北省承德市科技支撑计划项目,(编号:201801A010)

摘　　要：目的:检测非小细胞肺癌及正常对照组中PTEN、p-Akt及VEGF蛋白表达差异,探讨三者在肺癌发病中的作用机制。方法:利用免疫组织化学方法检测82例非小细胞肺癌和58例正常肺泡、56例正常支气管断端中VEGF、p-Akt及PTEN蛋白表达水平,分析三种蛋白表达差异与非小细胞肺癌患者临床病理特征的相关性。结果:p-Akt、VEGF在肺癌、肺鳞癌、肺腺癌中表达水平均显著高于正常对照组,且两者表达水平呈正相关;p-Akt水平与肿瘤直径、分化程度、淋巴结转移及临床分期存在相关性;VEGF水平与肿瘤分化程度、淋巴结转移及临床分期存在相关性;PTEN在肺癌、肺鳞癌、肺腺癌中表达均显著低于正常对照组;PTEN水平与肿瘤分化程度、淋巴结转移及临床分期存在相关性;6.PTEN、p-Akt表达呈负相关,PTEN、VEGF表达呈负相关。结论:p-Akt及VEGF对非小细胞肺癌发生、浸润及转移中起协同促进作用,PTEN起抑制作用。Objective: To detect the expression of PTEN,p-Akt and VEGF protein in tissue of non small-cell lung cancer group and normal control groups,and discuss the mechanism of these three proteins in the pathogenesis of non-small cell lung cancer. Methods: 82 cases of tissue samples with non small-cell lung cancer and fifty eight cases of tissue samples from alveolus health control,and fifty six cases of tissue samples from bronchus health control were collected. The expression levels of them was measured by immunohistochemistry method and analyzed the relationship with the clinical and pathological data. Results: The expression of p-Akt and VEGF protein in lung cancer group,squamous cell carcinoma of the lung group and lung adenocarcinoma group was significantly higher than which in double normal control groups(P<0.01).p-Akt and VEGF expression in lung tissue were positively correlated. The expression levels of p-Akt protein were related to tumor size,differentiation degree,clinical stage and lymph node metastasis. The expression levels of VEGF protein were related to differentiation degree ,clinical stage and lymph node metastasis. The expression levels of PTEN protein in lung cancer group,squamous cell carcinoma of the lung group and lung adenocarcinoma group is significantly lower than which in double normal control groups(P<0.01). The expression levels of PTEN protein were related to differentiation degree,clinical stage and lymph node metastasis. PTEN is negatively correlated with p-Akt and VEGF in tumor tissues. Conclusion: P-Akt and vascular endothelial growth factor play a synergistic role in the occurrence,invasion and metastasis of non-small cell lung cancer,and PTEN plays an inhibitory role.

关 键 词：非小细胞肺癌 肺泡 支气管 PTEN P-AKT VEGF 

分 类 号：R734.2[医药卫生—肿瘤]