两种耐药性颞叶癫痫模型海马硬化及苔藓纤维发芽的对比  

Comparative Study of Two Drug Resistant Temporal Lobe Epilepsy of Hippocampal Sclerosis and Mossy Fiber Sprouting

作　　者：许兰 王丽琨[1] 周鑫[1] 伍国锋[1] XU Lan;WANG Likun;ZHOU Xin;WU Guofeng(Emergency Department,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学附院急诊医学科

出　　处：《贵州医科大学学报》2019年第10期1145-1150,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金地区科学基金(81560222)

摘　　要：目的:用杏仁核电刺激慢点燃和匹罗卡品腹腔注射的方法建立多药耐药性颞叶癫痫模型,对比两种模型海马神经元坏死及苔藓纤维发芽(MFS)情况。方法:SD大鼠分为正常对照组、杏仁核敏感组、匹罗卡品敏感组、杏仁核耐药组及匹罗卡品耐药组,每组10只;后4组大鼠分别采用杏仁核电刺激慢点燃和匹罗卡品腹腔注射的方法建立颞叶癫痫模型、用苯巴比妥和苯妥英钠或卡马西平进行耐药性筛选,正常组大鼠不做任何处理;用HE染色观察海马组织神经元坏死情况,用硫化银染法(Timm’s)观察海马组织MFS情况。结果:4组模型大鼠海马CA3区神经元坏死比例均较正常对照组大鼠明显增多(P<0.05),且2组模型耐药大鼠高于各自的模型敏感组(P<0.05),但2种模型敏感组海马神经元坏死比例比较及2种模型耐药组神经元坏死比例比较,差异均无统计学意义(P>0.05);匹罗卡品敏感组及耐药组大鼠海马组织的MFS评分分别高于杏仁核敏感组及耐药组,差异具有统计学意义(P<0.05)。结论:匹罗卡品耐药模型MFS程度重,更加有利于癫痫耐药机制的研究。Objective:To establish a multi-drug resistant model of temporal lobe epilepsy by amygdale kindling or pilocarpine introperitonial injection,and to compare the characteristics the hippocampal sclerosis and mossy fiber sprouting in both models.Methods:SD rats were divided into control group,amygdaloid nucleus sensitive group,pilocarpine sensitive group,amygdaloid nucleus resistant group and pilocarpine resistant group.The four experiment groups were prepared for the model by chronic stimulation of amygaloid basal lateral nucleus and intraperitonial injection of pilocarpine.Then,phenobarbital and phenytoin or carbamazepine were used to screen for drug resistance,control group received no treatment.HE staining was used to observe the hippocampal sclerosis,the Timm,s staining was used to observe the mossy fiber sprouting condition.Results:CA3 region sclerosis proportion of all experiment groups significantly increased compared with control group(P<0.05),and mice of drug resistant models were higher than sensitive groups(P<0.05).The comparison between hippocamp al sclerosis proportion of both sensitive groups and comparison between hippocampal sclerosis proportion of both drug resistant groups showed no statistical significance(P>0.05).MFS scores of Pilocarpine sensitive group and drug resistant group were higher than that of amygdaloid nucleus sensitive and resistant group,difference showed statistical significance(P<0.05).Conclusions:The pilocarpine-induced models showed severe degree of MFS,which was beneficial for epilepsy drug mechanism research.

关 键 词：耐药性癫痫 杏仁核慢点燃模型 匹罗卡品癫痫模型 海马硬化 苔藓纤维发芽 

分 类 号：R742.1[医药卫生—神经病学与精神病学]