响应面法优化纤维素酶提取三叶青多糖的工艺研究  

Optimization of cellulase extraction process of polysaccharide from radix tetrastigmatis hemsleyani based on response surface method

作　　者：何文 李瀚鑫 Khudoyberdiev Ilkhomjon 王晰雯 叶春林[1] HE Wen;LI Hanxin;WANG Xiwen;YE Chunlin(School of Biological and Chemical Engineering,Zhejiang University ofScience and Technology,Hangzhou 310023,Zhejiang,China)

机构地区：[1]浙江科技学院生物与化学工程学院

出　　处：《浙江科技学院学报》2019年第5期349-356,共8页Journal of Zhejiang University of Science and Technology

基　　金：浙江省自然科学基金项目(LY17C020003)

摘　　要：为了优化三叶青多糖的酶法提取工艺,选用纤维素酶提取三叶青多糖。通过单因素试验法和响应面分析法,考察酶加量、酶解温度、酶解时间、水料比这4个因素对三叶青多糖提取率的影响。得到的最佳提取条件为:酶加量0.274%,酶解温度60.5℃,酶解时间62.4min,水料比25.8∶1(体积∶质量,mL/g),此条件下三叶青多糖的实际提取率为10.47%,与理论最佳提取率10.43%相比,相对误差为0.38%。此结果表明,运用响应面法优化得到的工艺条件准确可靠,能够真实地反映各因素对三叶青多糖提取率的影响,该提取工艺稳定合理、客观可行。In order to optimize the enzyme extraction process of polysaccharide from radix tetrastigmatis hemsleyani,the cellulase was selected to distill polysaccharide,investigating the influence of cellulase dosage,enzymolysis temperature,enzymolysis time and water to material ratio on extraction yield of polysaccharide by single factor experiment and response surface method. The optimum extraction conditions were 0.274% cellulose dosage at 60.5 ℃ for 62.4 min in a water to material ratio of 25.8∶1,where the actual extraction ratio of polysaccharide from radix tetrastigmatis hemsleyani was 10.47%,which was well matched with the predicted value of 10.43%,with the relative error of 0.38%. The process conditions optimized by response surface methodology are proven accurate and reliable,which can truly respond to the effects of various factors on the extraction rate of polysaccharide from radix tetrastigmatis hemsleyani. Hence the extraction process is stable,reasonable and feasible.

关 键 词：三叶青 多糖 响应面法 纤维素酶 提取工艺 

分 类 号：R284.2[医药卫生—中药学]