牛筋果中酚性化合物及其对DPPH自由基的清除活性  

Phenolic compounds from Harrisonia perforata (Blanco) Merr.and their DPPH radical scavenging antioxidant activity

作　　者：唐小涵 陈艳妮 汤红玉 李博婷 汪云松[1] 曾英[2] 郝小江[2] 邸迎彤[2] TANG Xiao-han;CHEN Yan-ni;TANG Hong-yu;LI Bo-ting;WANG Yun-song;ZENG Ying;HAO Xiao-jiang;DI Ying-tong(School of Chemical Science and Engineering,Yunnan University,Kunming 650091,China;State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute ofBotany,Chinese Academy of Sciences,Kunming 650201,China)

机构地区：[1]云南大学化学科学与工程学院,昆明650091 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明650201

出　　处：《天然产物研究与开发》2019年第10期1738-1744,共7页Natural Product Research and Development

基　　金：国家自然科学基金(3177011633)

摘　　要：本文对牛筋果Harrisonia perforata(Blanco)Merr.中的酚性成分及其抗氧化活性进行了研究。从牛筋果小枝中分离得到了14个酚性化合物,分别鉴定为:去甲丁香色原酮(1)、柴胡色原酮A(2)、5,7-dimethoxy-2-methylchromone(3)、2-hydroxymethylalloptaeroxylin(4)、羟苯乙酯(5)、苯丙酮(6)、cephafortin B(7)、去氢双松柏醇(8)、hawthornnin C(9)、hawthornnin D(10)、丁香树脂酚(11)、皮树脂醇(12)、4-ketopinoresinol(13)、罗汉松脂酚(14)。化合物1、3、5~10和12~13共10个化合物为首次从植物牛筋果中分离得到。使用DPPH法筛选了化合物的抗氧化活性,其中,化合物13和14抗氧化活性优于阳性对照(其EC 50值分别为53.88±5.34和32.67±3.96μM),并对其构效关系进行了初步探讨。该结果为牛筋果的深度开发利用提供了理论依据。The study was focus on the phenolic compounds of Harrisonia perforata(Blanco)Merr.and their antioxidant activity.Fourteen compounds were isolated from the branches of Harrisonia perforata(Blanco)Merr.by various column chromatography.Their structures were identified as noreugenin(1),saikochromone A(2),5,7-dimethoxy-2-methylchromone(3),2-hydroxymethylalloptaeroxylin(4),ethylparaben(5),propiophenone(6),cephafortin B(7),dehydroconiferyl alcohol(8),hawthornnin C(9),hawthornnin D(10),syringaresinol(11),medioresinol(12),4-ketopinoresinol(13)and matairesinol(14)based on ESI-MS,1H and 13 C NMR spectroscopic data.Among them,compound 1,3,5-10,and 12-13 were isolated from this plant for the first time.In addition,DPPH radical scavenging method was used to evaluate antioxidant activity,compound 13 and 14 showed strong DPPH free radical scavenging capacity.And then,we discussed the structure activity relationship of these phenolic compounds.The results provided a theoretical basis for deep development and utilization of Harrisonia perforata(Blanco)Merr.

关 键 词：牛筋果 化学成分 酚性化合物 抗氧化活性 构效关系. 

分 类 号：R284.2[医药卫生—中药学]