青龙衣中胡桃醌的提取及其抑菌活性研究  

Extraction and Antimicrobial Activity of Juglone from Pericarpium Juglandis

作　　者：李文君[1] 王成章[1] 刘辰婧 LI Wenjun;WANG Chengzhang;LIU Chengjing(Institute of Chemical Industry of Forest Products,CAF, National Engineering Lab. for Biomass Chemical Utilization, Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration,Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点实验室,江苏省生物质能源与材料重点实验室,江苏南京210042 [2]南京林业大学轻工与食品学院,江苏南京210037

出　　处：《林产化学与工业》2019年第5期95-100,共6页Chemistry and Industry of Forest Products

基　　金：江苏省生物质能源与材料重点实验室基本科研业务费项目(JSBEM-S-201810);中国林科院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017ZA004-2)

摘　　要：以青龙衣为原料,采用超声波辅助提取青龙衣中胡桃醌,通过单因素和正交试验优化得到最佳提取工艺条件;并以甲醇和商用YZN-Y500纳米银抗菌溶液作对照,考察最佳提取工艺条件下得到的质量浓度分别为0.20、0.02、0.01、0.005和0.0025g/L的青龙衣提取物样品溶液,对枯草芽孢杆菌、肺炎链球菌、金黄色葡萄球菌和表皮葡萄球菌的抑菌活性。研究结果表明:以甲醇为浸提溶剂,浸泡时间6h,液料比5∶1(mL∶g),超声波作用时间35min的最佳提取条件下胡桃醌得率0.246%,浸膏得率13.88%。青龙衣提取物溶液具有较强的抑菌活性,且溶液浓度越高,抑菌效果越明显;质量浓度为0.20、0.02g/L的青龙衣提取物样品溶液对4种菌的抑菌圈直径可达15~25mm,抑菌效果强于0.01g/L的商用纳米银溶液。Juglone was extracted from pericarpium juglandis by ultrasound-assisted method. The optimal conditions of extracting juglone were obtained by single experiment and orthogonal test as follows: soaking time was 6 h using methanol as solvent, ratio of liquid to material was 5∶1(mL∶ g), ultrasonic time was 35 min, and the yield of juglone was 0.246% and the yield of extract was 13. 88%. The antibacterial activities of extract on Bacillus subtilis, Streptococcus pneumoniae, Staphylococcus aureus and S. epidermidis of juglone was investigated by mass concentration of 0. 002 5 - 0. 20 g/ L, compared with commercial YZN-Y500 nano-silver. The results showed that the optimum extraction conditions was as follows: The antibacterial effect of pericarpium juglandis extract on the mass concentrations of 0.20g/ L and 0. 02 g/ L was stronger than that of commercial silver nanoparticles.

关 键 词：青龙衣 胡桃醌 超声波辅助 纳米银溶液 抑菌活性 

分 类 号：TQ35[化学工程]