多通道PCR荧光检测仪的光学系统设计  

Optical system design of multichannel PCR fluorescence detector

作　　者：谢欣茹 杨波[1] 潘帅 云竹溪 XIE Xinru;YANG Bo;PAN Shuai;YUN Zhuxi(School of Optical-electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学光电信息与计算机工程学院

出　　处：《光学技术》2019年第5期531-534,640,共5页Optical Technique

基　　金：国家重点研发计划(2016YFB0402000)

摘　　要：针对目前实时定量PCR荧光检测仪系统中存在的检测效率低、体积大等问题,提出了一种基于传统单通道共聚焦型光路改善组件的方法,使激发光路和荧光收集光路共用一个大孔径透镜,并在该透镜的下方围绕多个激发光准直光路,同时在荧光收集光路中添加多个二向色镜,实现多通道荧光的同时检测。运用Code V软件设计出准直透镜和聚焦镜,将透镜数据导入到Lighttools软件中进行仿真模拟,结果表明,光学系统解决了现有实时定量PCR荧光检测仪中存在的问题,检测效率是原系统的2倍以上,体积比传统单通道集成的结构小2倍以上。Aiming at the problems of low detection efficiency and large volume in the current real-time quantitative PCR fluorescence detector system, a method for improving components based on the original single-channel confocal optical path is proposed. The excitation light path and the fluorescence collection light path share a large-diameter lens, and a plurality of excitation light collimation optical paths are arranged under the lens, and a plurality of dichroic mirrors are added to the fluorescence collection optical path to realize simultaneous detection of multi-channel fluorescence. Using the Code V software to design the collimating lens and the focusing lens, the lens data was imported into the Lighttools software for simulation. The results show that the optical system solves the problems existing in the existing real-time quantitative PCR fluorescence detector. The detection efficiency is more than 2 times that of the original system, while effectively reducing the volume of the fluorescence detection device, and improving the cost performance of the instrument.

关 键 词：应用光学 PCR荧光检测仪 多通道 二次曲面 光学系统设计 

分 类 号：TP394.1[自动化与计算机技术—计算机应用技术] TH691.9[自动化与计算机技术—计算机科学与技术]