L型氨基酸转运载体1在结直肠癌组织高表达并与肿瘤转移相关  

High expression of L-type amino acid transporter 1 is associated with colorectal cancer metastasis

作　　者：王昕[1] 朱瑞平[2] 王华桥 朱雄斌 吴旭初[1] 周芬芳 卢作鹏[1] 陈志芬[2] 冯茂辉[3] Wang Xin;Zhu Ruiping;Wang Huaqiao;Zhu Xiongbin;Wu Xuchu;Zhou Fenfang;Lu Zuopeng;Chen Zhifen;Feng Maohui(Department of Gastrointestial Surgery,Tongcheng People’s Hosptial,Tongcheng 437400,China;Department of Gastroenterology,the Hubei Clinical Center&Key laboratory of intestinal&Colorectal Diseases,Zhongnan Hospital of Wuhan University,Wuhan 430071,China;Department o f Gastrointestinal Surgery,Zhongnan Hospital ,Clinical Cancer Study Center of Hubei Provence,Key Laboratory of Tumor Biological Behavior of Hubei Provence,Clinical Medical Research Center of Peritoneal Cancer of Wuhan,Wuhan University,Wuhan 430071,China)

机构地区：[1]通城县人民医院胃肠外科,437400 [2]武汉大学中南医院消化内科肠病湖北省重点实验室,430071 [3]武汉大学中南医院胃肠外科武汉市腹膜癌临床医学研究中心湖北省肿瘤医学临床研究中心肿瘤生物学行为湖北省重点实验室,430071

出　　处：《中华实验外科杂志》2019年第10期1854-1856,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81770283);湖北省卫生计生委科研基金(WJ2017M249);武汉市腹膜癌临床医学研究中心资助项目(2015060911020462).

摘　　要：目的探讨L型氨基酸转运载体1(LAT1)在结直肠癌中的表达及其作用.方法利用GEPIA数据库分析LAT1在结直肠癌中的表达.在60对新鲜人体结直肠癌组织、癌旁组织中经实时定量反转录聚合酶链反应(RT-qPCR)、免疫组织化学分析方法检测LAT1基因在mRNA和蛋白水平的表达.分析结直肠癌组织LAT1表达量与患者临床资料的关系.应用R3.5.0统计软件分析.采用Student t检验分析CRC组织和癌旁正常组织中LAT1 mRNA的差异表达.应用Fisher精确检验来评估LAT1蛋白表达与CRC的临床病理特征之间的相关性.结果 GEPIA数据库分析表明LAT1 mRNA在结直肠癌中高表达.RT-qPCR表明LAT1 mRNA在结直肠癌组织(4.448±2.276)中的表达明显较癌旁正常组织(1.604±0.947)中升高(P<0.01).同时,免疫组织化学实验表明在60例结直肠癌组织中,46例高表达LAT1蛋白,14例低表达LAT1蛋白;而在60例相应的癌旁组织中,48例低表达LAT1蛋白,12例高表达LAT1蛋白,采用Fisher精确检验表明结直肠癌组织中高表达LAT1蛋白(P<0.01).此外,结合临床特征分析表明LAT1蛋白高表达与淋巴结转移(P<0.05)、肝转移(P<0.05)和TNM分期(P<0.05)明显相关.结论 LAT1在结直肠癌中高表达,且其表达量与肿瘤转移明显相关.Objective To investigate the expression and significance of L-type amino acid transporter 1 (LAT1) in human colorectal cancer. Methods Sixty pairs of colorectal cancer tissues and paracancerous tissues were collected from sixty advanced colorectal cancer (CRC) patients for detecting LAT1 expression. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and immunohistochemical analysis were used to analyze the expression of LAT1 in colorectal cancer tissues and adjacent normal tissues. In addition, we also study the relationship between LAT1 expression and the clinical features of sixty patients with colorectal cancer. Results Bioinformatics analysis through GEPIA database suggested that LAT1 mRNA expression was elevated in colorectal cancer. We also observed markedly increased LAT1 mRNA and protein expression in colorectal cancer compared with adjacent normal tissues (P<0.01). In addition, we found high expression of LAT1 protein was closely related to lymphatic metastasis (P<0.05), liver metastasis (P<0.05), and TNM stage (P<0.05). Conclusion LAT1 expression is increased in colorectal cancer and asscciated with tumor metastasis.

关 键 词：L型氨基酸转运载体1 结直肠癌 淋巴结转移 肝转移 TNM分期 

分 类 号：R735.3[医药卫生—肿瘤]