Ca2+、Mg2+对剩余污泥自培养产酶影响  

Ca2+,Mg2+ affecting enzyme producing in excess activated sludge self-culturing

作　　者：李婧 南亚萍 袁林江 邹建旻 王龄侦 LI Jing;NAN Ya-ping;YUAN Lin-jiang;ZOU Jian-min;WANG Ling-zhen(School of Environmental and Municipal Engineering,Xi’an University of Architecture and Technology,Shaanxi Key Laboratory of Environmental Engineering,Key Laboratory of Northwest Water Resource,Environment and Ecology,MOE,Xi’an 710055,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院陕西省环境工程重点实验室西北水资源与环境生态教育部重点实验室

出　　处：《应用化工》2019年第10期2363-2368,2373,共7页Applied Chemical Industry

基　　金：国家自然科学基金项目(51308440);国家自然科学基金项目(50878180)

摘　　要：为提高剩余污泥投加复合菌后产水解酶效果,探讨了添加Ca^2+、Mg^2+对剩余污泥自培养产酶中污泥水解的影响。结果表明,未加Ca^2+、Mg^2+的空白组,蛋白酶和淀粉酶活力均呈现第1d上升至最大值,后持续降低的趋势。添加0.03mol/L Ca^2+组的蛋白酶活力第1d快速升至246.14U/mL,后持续上升,并在第7d酶活力达最大值340.07U/mL,蛋白酶活力最大值较空白组增加了53.94%;淀粉酶活力在第1d快速升至351.02U/mL后快速下降,第7d酶活力为70.79U/mL,淀粉酶活力最大值较空白组增加了51.93%,表明添加0.03mol/L Ca^2+可以提高灭菌污泥自培养产酶效果。而添加0.06mol/L Ca^2+组酶活力呈下降趋势,表明高浓度Ca^2+对产酶有抑制作用。添加0.03mol/L或0.06mol/L Mg^2+,不能提高产酶效果,但能维持酶活力稳定。添加合适浓度Ca^2+、Mg^2+能强化剩余污泥产酶过程中污泥水解效果,促进污泥上清液中有机物水解。In order to improve the hydrolytic enzymes production of excess sludge.The influence of adding Ca^2+ and Mg^2+ on sludge connection in the enzyme production of residual sludge were investigated.The result showed that the protease activity and the amylase activity increased the max on the 1th d and then decreased continuously that in controlled samples without metal ions.In the samples with 0.03 mol/L Ca^2+,the protease activity increased to 246.14 U/mL rapidly on the 1th day,then continued to rise and reached 340.07 U/mL the max on the 7th d,which increased 53.94% compared with the controlled samples.The amylase activity increased to 351.02 U/mL rapidly on the 1st day and decreased to 70.79 U/mL on the 7th d,which increased 51.93% compared with the controlled samples.It indicated that the 0.03 mol/L Ca^2+ could improve the hydrolase activity producing efficiency in the sterilized sludge self-culturing.But when adding 0.06 mol/L Ca^2+ in excess sludge,the enzyme activity did not increase,just showed a downward trend,indicating that high concentration of Ca^2+ inhibited enzyme production.In the Mg^2+ sample,neither 0.03 mol/L nor 0.06 mol/L Mg^2+ could improve the production of hydrolase,but could maintain the stability of hydrolase activity.Appropriate concentrations of Ca^2+ and Mg^2+ could enhance the hydrolysis in excess sludge self-culturing,which could promote the organic compounds hydrolysis of excess sludge supernatant.

关 键 词：蛋白酶 淀粉酶 金属离子 污泥水解 自培养 

分 类 号：TQ925[轻工技术与工程—发酵工程] TQ033