茶树越冬芽萌展期碳氮代谢动态及相关基因分析  

Analysis of dynamic changes of carbon and nitrogen metabolism and related gene expression in overwintering axillary bud during germination period of tea plant(Camellia sinensis)

作　　者：吴琼 刘丹丹[1] 阮旭 沈季雪 徐奕鼎 王文杰 张正竹 夏恩华[2] WU Qiong;LIU Dandan;RUAN Xu;SHEN Jixue;XU Yiding;WANG Wenjie;ZHANG Zhengzhu;XIA Enhua(Tea Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031;State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036)

机构地区：[1]安徽省农业科学院茶叶研究所,合肥230031 [2]安徽农业大学省部共建茶树生物学与资源利用国家重点实验室,合肥230036

出　　处：《安徽农业大学学报》2019年第4期559-563,共5页Journal of Anhui Agricultural University

基　　金：安徽省农业科学院人才发展专项(17F0812);安徽省农业科学院院所共建团队项目(18C0832);安徽省重点研究与开发项目(1704a07020065);现代农业产业技术体系建设专项资金项目(CARS-19)共同资助

摘　　要：以早生型茶树品种"皖茶4号"(原名红旗1号)为材料,研究茶树越冬腋芽(Axillary bud,AB)与毗邻成熟叶(下称母叶,Matureleaf,ML)萌展期的碳氮代谢动态变化,同时根据腋芽全转录组数据分析相关基因的表达情况。结果表明,不同发育时期的腋芽与母叶中的淀粉、可溶性糖、NSC及糖/淀粉比值差异极显著(P<0.01);腋芽与母叶的可溶性蛋白含量差异极显著(P<0.01);腋芽中SPS酶活性在3个发育时期差异极显著(P<0.01),母叶中SPS、ATP、ALT酶活性在不同发育时期差异极显著(P<0.01);通过比较腋芽各生育期的基因表达情况,发现有8个与碳氮代谢相关的基因表达差异显著(P<0.05)。The dynamic changes of carbon and nitrogen metabolism in overwintering axillary buds(AB)and adjacent mature leaves(ML)of tea plants during the germination period were detected using the early-generation tea tree variety"Wancha 4"(formerly known as"Hongqi 1")as the material.The expression of related genes in axillary buds was also analyzed based on the total transcriptome data.The results showed that:from winter dormancy to bud flush stages,the ratios of starch,soluble sugar,NSC and sugar/starch in axillary buds and mature leaves at different developmental stages were extremely significantly different(P<0.01);the difference in soluble protein content between axillary buds and mature leaves was extremely significant(P<0.01);the activity of SPS in the three developmental stages was extremely significant(P<0.01);the activities of SPS,ATP and ALT in the mother leaves were significantly different at different developmental stages(P<0.01).It was found that there are significant differences in the expression of 8 genes involved in carbon and nitrogen metabolism(P<0.05).

关 键 词：茶树 越冬芽 碳氮代谢 

分 类 号：S571.1[农业科学—茶叶生产加工]