PD-1/PD-L1在结直肠癌组织中的表达及其临床意义  

Expressions and clinical significance of PD-1/PD-L1 in colorectal carcinoma tissues

作　　者：刘宗航[1] 史丽芸 郑立锋[1] 李现雷 LIU Zong-hang;SHI Li-yun;ZHENG Li-feng;LI Xian-lei(Department of General Surgery,Nanjing Jiangbei People's Hospital,Nanjing,Jiangsu 210048 ,China)

机构地区：[1]南京江北人民医院普外科,江苏南京210048 [2]南京江北人民医院病理科,江苏南京210048

出　　处：《中国临床研究》2019年第10期1364-1367,共4页Chinese Journal of Clinical Research

摘　　要：目的探讨程序性死亡因子(PD)-1/PD配体1(PD-L1)蛋白在结直肠癌组织中的表达水平及其与患者临床病理特征的关系。方法收集2015年1月至2018年6月80例结直肠癌手术患者的术后石蜡组织切片及临床资料。采用免疫组化法检测肿瘤组织中的PD-1和PD-L1蛋白的表达情况,分析PD-1和PD-L1与结直肠癌患者临床病理特征的关系。结果PD-1和PD-L1蛋白在结直肠癌组织中的阳性表达率分别为112%和538%。PD-1蛋白在不同临床病理特征患者间的表达差异均无统计学意义(P>0.05)。在有淋巴结转移、肿瘤T分期高、分化程度低以及病理分期高的患者中,PD-L1蛋白阳性表达率明显高于无淋巴结转移、肿瘤T分期低、分化程度高以及病理分期低的患者,差异有统计学意义(P<0.05,P<0.01)。配对资料两因素关联性的χ2检验结果显示,结直肠癌组织中PD-L1的阳性表达(阳性表达率53.8%)与PD-1表达的缺失(阳性表达率11.2%)存在显著关联(χ^2=32.93,P<0.01)。结论PD-L1在结直肠癌组织中高表达,并与患者的不良临床病理特征有关,以PD-1/PD-L1信号通路为靶点的免疫治疗有望成为结直肠癌的潜在治疗方案。Objective To investigate the expression levels of programmed death factor 1 ( PD-1 ) and programmed death ligand 1 ( PD-L1 ) proteins in colorectal carcinoma ( CRC ) tissues and the relationship between them and the clinicopathological features of patients. Methods The paraffin embedded tumor tissues after operation and the clinical data of 80 CRC patients from January 2015 to June 2018 were collected. The protein expressions of PD-1 and PD-L1 in tumor tissues were detected by immunohistochemistry method. The relationship between PD-1/ PD-L1 expression and clinicopathological characteristics was analyzed. Results The positive rates of PD-1 and PD-L1 in colorectal cancer tissues were 11. 2% and 53. 8%, respectively. There was no significant difference in the expression of PD-1 protein in the patients with different clinicopathological characteristics (P > 0. 05), but the positive expression rates of PD-L1 protein in patients with lymph node metastasis, high T stage, low degree of differentiation and high pathological stage were significantly higher than those in patients without lymph node metastasis, low T stage, high degree of differentiation and low pathological stage (P <0. 05 ,P< 0. 01 ). The results of X^2 test of paired data showed that there was a significant correlation between the expression of PD-L1 ( positive expression rate = 53. 8%) and the loss of PD-1 expression ( positive expression rate = 11. 2%) in colorectal cancer (X^2= 32. 93 ,P < 0. 01). Conclusions The high expression of PD-L in colorectal cancer tissues is related to the adverse clinicopathological characteristics of patients. Immunotherapy targeting PD-1/PD-L1 signaling pathway is expected to become a potential treatment for colorectal cancer.

关 键 词：结直肠癌 程序性死亡因子1 程序性死亡因子配体1 临床病理特征 

分 类 号：R735.3[医药卫生—肿瘤]