gC1qR基因致胃癌细胞凋亡的作用及其机制研究  

Effect of gC1qR gene on apoptosis of gastric cancer cells and its mechanism

作　　者：侯进军 杨洋 朱成海 高玲娟[3] HOU Jin-jun;YANG Yang;ZHU Cheng-hai;Gao Ling-juan(Department of Clinical Laboratory,Maternal and Child Health Hospital of Qixia District,Nanjing,Jiangsu 210028,China)

机构地区：[1]南京市栖霞区妇幼保健院检验科,江苏南京210028 [2]南京市栖霞区妇幼保健院内科,江苏南京210028 [3]南京市妇幼保健院检验科,江苏南京210004

出　　处：《中国临床研究》2019年第10期1408-1411,共4页Chinese Journal of Clinical Research

基　　金：2016年度南京市医学科技发展项目(YKK16292)~~

摘　　要：目的观察球状C1q受体(gC1qR)对胃癌细胞凋亡的影响及其可能的机制。方法收集2017年1月至2018年6月就诊的12例胃癌患者的胃癌组织及其对应的癌旁组织,其中年龄(780±65)岁的老年胃癌组6例,年龄(483±36)岁的非老年胃癌组6例;另选由杭州赫贝生物技术提供的胃癌细胞株MKN-28(人胃癌高分化腺癌细胞株)。对患者的胃癌组织和癌旁组织,采用Real-time PCR方法检测其幽门螺杆菌(Hp)DNA表达水平;免疫印迹法(Western blot)检测gC1qR蛋白的表达;免疫组化方法检测gC1qR蛋白的表达及定位情况。通过构建gC1qR基因载体转染胃癌细胞株MKN-28,采用流式细胞技术检测gC1qR过表达载体组、空载体组和空白对照组细胞凋亡情况。结果胃癌组织Hp感染率显著高于癌旁正常组织(75.0%vs 33.3%,P=0.010)。非老年胃癌组织和老年胃癌组织gC1qR蛋白的相对表达量均明显低于癌旁正常组织(0.37±0.03 vs 0.74 ± 0.05,P<0.01;0.22 ± 0.02 vs 0.76 ± 0.04,P<0.01);且老年胃癌组显著低于非老年胃癌组(P<0.01)。gC1qR过表达载体组细胞凋亡率40.6%,空载体组8.7%,空白对照组62%,差异有统计学意义(P<0.01)。结论gC1qR基因具有显著诱导胃癌细胞凋亡的作用,其有望成为胃癌诊断、治疗及预后的判断新靶标。Objective To observe the effect of globular Clq receptor ( gClqR) on apoptosis of gastric cancer cells and its possible mechanism. Methods The gastric cancer tissues and their corresponding adjacent tissues of 12 patients with gastric cancer treated from January 2017 to June 2018 were collected. Among them, elderly group aged ( 78.0±6.5 ) years,non-elderly group aged (48.3±3.6) years,each group with 6 cases. The gastric cancer cell line MKN-28 ( human gastric cancer highly differentiated adenocarcinoma cell line) provided by Hangzhou Hebei Biotechnology was also selected. PCR mothed was used to detect helicobacter pylori ( Hp) DNA level;Western blot analysis was applied to detect the expression of gClqR protein;immunohistochemical method was used to detect the protein expression and its localization of gClqR in gastric tissues and paracancerous tissues. The gClqR gene vector was constructed and transfected into gastric cancer cell line MKN-28;flow cytometry was used to detect the cells apoptosis in gClqR overexpression vector group, blank vector group and blank control group. Results The Hp infection rate of gastric cancer tissues was significantly higher than that of adjacent normal tissues (75. 0% vs 33. 3%,P = 0. 010). The relative expression of gClqR protein in non-elderly gastric cancer tissues and elderly gastric cancer tissues was significantly lower than that in adjacent normal tissues (0.37±0.03 vs 0.74±0.05,P<0.01;0.22±0.02 vs 0.76±0.04,P<0.01),respectively;and the relative expression of gClqR protein in elderly group was significantly lower than that in non-elderly group ( P < 0. 01 ). The apoptosis rate of gClqR overexpression vector group was 40. 6%, empty vector group was 8. 7%, blank control group was 6. 2%, the difference was statistically significant ( P < 0. 01 ). Conclusion The gClqR gene can significantly induce apoptosis of gastric cancer cells, and it is expected to be a new target for the diagnosis, treatment and prognosis of gastric cancer.

关 键 词：胃癌 球状C1q受体 凋亡 幽门螺杆菌 

分 类 号：R735.2[医药卫生—肿瘤]