金铁锁三萜皂苷合成生物学研究进展  

Research Progress of the Synthetic Biology of Triterpenoid Saponins from Psammosilene Tunicoides

作　　者：李畏娴 张爱丽[2] 钱子刚[2] 陈洁 孙辉 陈永刚 刘幸 LI Weixian;ZHANG Aili;QIAN Zigang;CHEN Jie;SUN Hui;CHEN Yonggang;LIU Xing(The Third People' s Hospital of Kunming,Kunming,Yunnan,China 650041;Yunnan University of Traditional Chinese Medicine,Kunming,Yunnan,China 650500)

机构地区：[1]云南省昆明市第三人民医院,云南昆明650041 [2]云南中医学院,云南昆明650500

出　　处：《中国药业》2019年第21期9-12,共4页China Pharmaceuticals

基　　金：云南省科技计划中医联合面上项目[2017FF117-030]

摘　　要：金铁锁是国家二级保护珍稀濒危药用植物,三萜皂苷类化合物是其主要活性成分。目前,利用合成生物学构建酿酒酵母细胞工厂高效生产药用植物中有效化合物的研究已在逐步完善,为实现微生物发酵生产药用中药萜类有效成分提供了基础。掌握金铁锁有效成分三萜皂苷的生物合成途径,是进行金铁锁定向育种和利用合成生物学进一步开发人工细胞工厂发酵生产金铁锁总皂苷等活性成分的基础。该文利用合成生物学的方法,在酿酒酵母细胞中进行金铁锁三萜皂苷的生物合成途径异源重建,以解析金铁锁有效成分生物合成的途径。Psammosilene tunicoides is a rare and endangered medicinal plant of the national second-class protection,and its main active ingredient is triterpenoid saponins. At present,the use of synthetic biology to construct Saccharomyces cerevisiae cell factories to efficiently produce effective compounds in medicinal plants has been gradually improved,which provides a basis for the realization of microbial fermentation to produce the active ingredient of the medicinal Chinese terpenoids. Mastering the biosynthetic pathway of triterpenoid saponins is the foundation for directive breeding of Psammosilene tunicoides and fermenting and producing the active ingredient of Psammosilene tunicoides by furthermore developing the artificial cell factories using synthetic cell biology. For realizing the biosynthetic pathway analysis of the active ingredient of Psammosilene tunicoides,this research uses the method of synthetic biology to make heterologous reconstruction of the biosynthetic pathway in triterpenoid saponins from Saccharomyces cerevisiae.

关 键 词：金铁锁 三萜皂苷 合成生物学 生物合成途径 酿酒酵母 进展 

分 类 号：R932[医药卫生—生药学] R284[医药卫生—药学]