质谱技术在糖尿病血液非酶促糖化蛋白分析中的应用进展  

Application of Mass Spectrometry in Analysis of Non-Enzymatic Glycation Proteins in Diabetic Blood

作　　者：李卫峰 阎德文[2] 金宇[2] 李海燕[2] 马民[1] 吴正治[1,2,3] LI Wei-Feng;YAN De-Wen;JIN Yu;LI Hai-Yan;MA Min;WU Zheng-Zhi(Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China;The First Affiliated Hospital of Shenzhen University, Shenzhen 518020, China;Shenzhen Institute of Gerontology, Shenzhen 518020, China)

机构地区：[1]暨南大学中西医结合博士后流动站,广州510632 [2]深圳大学第一附属医院,深圳,518020 [3]深圳市老年医学研究所,深圳518020

出　　处：《分析化学》2019年第11期1732-1741,共10页Chinese Journal of Analytical Chemistry

基　　金：深圳市医疗卫生三名工程项目(No.SZSM201612049);国家自然科学基金项目(No.81574038);深圳市学科布局项目(No.JCYJ20170412161254416)资助~~

摘　　要：糖尿病是一种以高血糖为特征的代谢紊乱性疾病,长期的高血糖生理环境会导致蛋白质非酶促糖化程度加重,进而引发一系列病理变化。因此,蛋白质非酶促糖化引起了研究者的关注。质谱技术因具有超高灵敏度、低检出限和多组分同时分析的优势,成为蛋白质定性和定量分析不可或缺的有力工具,已被应用于非酶促蛋白翻译后修饰信息的鉴定和定量分析。本文主要对蛋白质非酶促糖化的产生原理及质谱在血液中非酶促糖化蛋白的修饰鉴定分析的研究进展进行了评述,并对该领域的发展趋势进行了展望。Diabetes mellitus is a metabolic disorder disease characterized by hyperglycemia. Long-term physiological environment of hyperglycemia will aggravate the degree of non-enzymatic glycation proteins. Because the abnormal changes of non-enzymatic process will cause a series of pathological changes, non-enzymatic glycation proteins have attracted researchers' attention. Mass spectrometry is an indispensable and powerful analytical tool for qualitative and quantitative analysis of proteins due to its ultra-high sensitivity, excellent detection limit, and multi-component simultaneous analysis, and has been applied to the identification and qualification of non-enzymatic glycation protein. Herein, the principle of non-enzymatic proteins and the research progress of mass spectrometry in the identification and analysis of glycated plasma proteins and hemoglobin in blood were reviewed, and the development trend of this field was prospected.

关 键 词：质谱 非酶促糖化 蛋白质 糖尿病 评述 

分 类 号：O657.63[理学—分析化学] R587.1[理学—化学]