LAP^+CD4^+T细胞在肝癌微环境中的分布特点及意义  

Distribution characteristics of LAP^+CD4^+T cells in the tumor microenvironment of hepatocellular carcinoma and its significance

作　　者：陈景森 欧希[2] 赵洋[3] 余光银[3] 刘晓平[2] CHEN Jingsen;OU Xi;ZHAO Yang;YU Guangyin;LIU Xiaoping(Breast Department,Affiliated Shenzhen Maternity & Child Healthcare Hospital,Southern Medical University,Shenzhen 518028,Guangdong,China;Department of Hepatopancreatobiliary surgery,Peking University Shenzhen Hospital,Shenzhen 518036,Guangdong,China;Department of Pathology,Pe-king University Shenzhen Hospital,Shenzhen 518036,Guangdong,China.)

机构地区：[1]南方医科大学附属深圳妇幼保健院乳腺科,广东深圳518028 [2]北京大学深圳医院肝胆胰外科,广东深圳518036 [3]北京大学深圳医院病理科,广东深圳518036

出　　处：《岭南现代临床外科》2019年第5期528-532,共5页Lingnan Modern Clinics in Surgery

基　　金：深圳市科技计划项目(JC200903180670A及201103023)

摘　　要：目的研究肝癌患者肿瘤组织中LAP^+CD4^+T细胞的分布特点,探讨LAP^+CD4^+T细胞在肝癌发生发展中的作用。方法抽取北京大学深圳医院2011年10月到2012年12月期间28例肝癌患者的手术肝组织标本与28例因良性病变行肝切除的正常肝组织标本,采用双重酶标免疫组化染色法检测LAP^+CD4^+T细胞在肝癌组织、癌旁组织及正常肝组织中的分布特点,并取单个高倍视野平均数进行半定量分析。结果肝癌患者肿瘤组织中LAP定位于CD4^+T细胞的胞浆和胞膜上;正常肝组织及癌旁组织中LAP^+CD4^+T细胞以散在方式分布,而在癌组织中多数以簇丛状方式聚集分布于肿瘤间质内,与CD4^+T细胞伴行并与之紧密接触。肝癌组、癌旁组以及正常对照组中LAP^+CD4^+T细胞单个高倍视野平均数分别为(11.25±3.00)、(5.75±1.00)及(2.61±0.83);肝癌组与癌旁组比较、肝癌组与正常对照组比较、癌旁组与正常对照组比较,三组比较差异均有统计学意义(P=0.000,P=0.000,P=0.000,P<0.05)。结论肝癌患者肿瘤组织中LAP定位于CD4^+T细胞的胞浆和胞膜上,肝癌微环境中LAP^+CD4^+T细胞明显聚集,多以簇丛状方式分布,与其它淋巴细胞紧密接触,分布数量较癌旁组织及正常肝组织均增多,提示LAP^+CD4^+T细胞在肝癌微环境中浸润,并有可能通过细胞接触的方式抑制肿瘤局部免疫,促使肝癌细胞逃避免疫监视。Objective To study the distribution characteristics of LAP^+CD4^+T cells in Tumor tissue of hepatocellular carcinoma(HCC)and to explore the role of LAP^+CD4^+T cells in occurrence and development of HCC. Methods Tumor tissues and peri-tumor tissues from 28 patients with HCC during October 2011 to December 2012,as well as hepatic tissues from 28 HBV-infected patients with benign lesions were subjected to immunohistochemical analysis with double staining for LAP and CD4,and the average number of the LAP^+CD4^+T cells in each visual field was quantified. Results The average number of LAP^+CD4^+T cells underling an individual high magnification vision in HCC tissues were significantly higher than those of control tissues(11.25±3.00 vs 2.61±0.83,P=0.000). The average number of LAP^+CD4^+T cells underling an individual high magnification vision in peri-cancer tissues were significantly higher than those of control tissues(5.75±1.00 vs 2.61±0.83,P=0.000). This significant difference was also viewed in HCC tissues and peri-cancer tissues(11.25±3.00 vs 5.75±1.00,P=0.000). Conclusions LAP^+CD4^+T cells accumulate in the tumor microenvironment of HCC. LAP^+CD4^+T cells could accelerate the immune escape by suppressing the local immune system in the development of HCC. LAP^+CD4^+T cells would play a role in negatively regulating through cell to cell contact.

关 键 词：LAP^+CD4^+T细胞 肿瘤微环境 免疫组化 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]