大鼠局灶性脑缺血再灌注损伤后MBP、MAGLingo-1的表达及丁苯酞对其的影响  

The expression of the MBP/MAG/Lingo-1 and the effect of dl-3-n-butyphthalide in rats after focal cerebral ischemia-reperfusion injury

作　　者：白小梅 李正仪[2] BAI Xiaomei;LI Zhengyi(Department of Pathology,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)

机构地区：[1]西安交通大学第二附属医院病理科,陕西西安710004 [2]西安交通大学第一附属医院神经内科,陕西西安710061

出　　处：《中风与神经疾病杂志》2019年第10期868-872,共5页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨大鼠局灶性脑缺血再灌注损伤后脑组织中髓鞘碱性蛋白(MBP)、髓鞘相关糖蛋白(MAG)和Nogo受体作用蛋白-1(Lingo-1)表达情况及丁苯酞(NBP)对其的影响。方法 72只大鼠随机分为对照组、模型组和药物组。通过阻塞大鼠左侧大脑中动脉造成脑缺血2 h后再灌注1 d、3 d、5 d、7 d,建立大鼠局灶性脑缺血再灌注损伤模型。药物组大鼠腹腔注射丁苯酞20 mg/(kg·d),2次/d;对照组注射生理盐水。用免疫组化法检测大鼠缺血侧脑皮质MBP、MAG和Lingo-1的表达并测定相应灰度值。结果模型组、药物组较对照组MBP表达减少,MAG及Lingo-1表达增多;药物组较模型组MBP表达增多,MAG表达减少,差异均有统计学意义(P <0. 05);药物组和模型组Lingo-1表达差异无统计学意义(P> 0. 05)。结论脑缺血再灌注诱发脑皮质髓鞘损伤,丁苯酞对受损髓鞘有一定保护作用。Objective To investigate the expression of myelin basic protein( MBP),myelin associated glycoprotein( MAG) and Nogo recepter-interacting protein-1( Lingo-1) in rat after focal cerebral ischemia-reperfusion injury and to explore the effect of dl-3 n-butylphthalide( NBP) on the 3 different proteins. Methods 72 adult rats were divided into sham group,model group and drug group. The animal model of middle cerebral artery occlusion( MCAO) was performed to make cerebral ischemia sustained for 2 h on rats and kept reperfusion for 1 d,3 d,5 d or 7 d. In the drug group,rats received intraperitoneal injection of NBP with the dose of 20 mg/( kg·d) and the frequency of twice a day. In the sham group,rats received intraperitoneal injection of saline. Immunohistochemistry was used to detect the expressions of MBP,MAG and Lingo-1 in the rats’ brains. Results In the model group and drug group,the expression of MBP decreases,MAG and Lingo-1 increases in rats compared with the sham group. In the drug group,the expression of MBP increases,MAG decreases in rats compared with the model group,the differences were statistically significant( P < 0. 05). The difference of the expression of Lingo-1 between drug group and model group was not statistically significant( P > 0. 05). Conclusion Cerebral ischemia-reperfusion can cause the damage on myelin in the cortical area of rats and NBP may have protective effect on impaired myelin.

关 键 词：缺血再灌注 髓鞘 MBP/MAG/Lingo-1 丁苯酞 

分 类 号：R743.3[医药卫生—神经病学与精神病学]