血清sICAM-1水平及ICAM-1基因K469E多态性与脑小血管病关系研究  

Associations of sICAM-1 serum levels and ICAM-1 K469E gene polymorphism with cerebral small vascular disease

作　　者：王利军[1] 王建平[1] 牛延良[1] 贺刚锐[1] WANG Lijun;WAHG Jianping;NIU Yanliang(Department of Neurology,The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第五附属医院神经内科

出　　处：《中风与神经疾病杂志》2019年第10期902-905,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨血清sICAM-1水平及ICAM-1基因K469E多态性与脑小血管病之间的关系。方法选择脑小血管病(CSVD)患者158例为病例组,选择健康体检者150例为对照组。利用ELISA法检测两组血清sICAM-1水平,并对比分析;利用PCR-RFLP法对两组ICAM-1基因K469E多态性位点进行检测和分析。结果(1) CSVD组sICAM-1血清水平明显高于对照组(P <0. 001);(2) CSVD组EE基因型和E等位基因频率明显高于对照组(χ^2=6. 435,P=0. 011;χ^2=9. 049,P=0. 003)。Logistic回归分析校正后,CSVD组与对照组之间ICAM-1基因EE型分布差异仍有统计学意义(OR=2. 649,P=0. 014)。结论血清sICAM-1可以作为CSVD的生物学标志物;ICAM-1 K469E基因EE基因型与CSVD具有相关性,提示ICAM-1 K469E基因多态性可能是CSVD发病的遗传学危险因素。Objective To investigate the associations of sICAM-1 serum levels and ICAM-1 K469 E gene polymorphism with cerebral small vascular disease. Methods A total of 158 patients with cerebral small vascular disease were selected as the observations group and 150 healthy people enrolled as the control group. Serum level of sICAM-1 was tested by ELISA. PCR-RFLP was used to detect and analyze the polymorphism of ICAM-1 K469 E gene. Results( 1) Serum level of sICAM-1 was significantly higher in CSVD group than that in control group( P < 0. 001).( 2) The frequencies of EE genotype and E allele of K469 E in CSVD group were significantly higher than that in control group(χ^2= 6. 435,P = 0. 011;χ^2= 9. 049,P = 0. 003). After correction by conditional logistical regression,the frequency distribution difference of the EE genotype between the CSVD group and controls remained statistically significant( OR = 2. 649,P = 0. 014). Conclusions Serum level of sICAM-1 can be used as biological marker of CSVD. The EE genotype of ICAM-1 K469 E gene is correlated with CSVD,the polymorphism of ICAM-1 K469 E gene may be a genetic risk factor of CSVD.

关 键 词：脑小血管病 可溶性细胞间粘附分子1 细胞间粘附分子1 基因多态性 

分 类 号：R743[医药卫生—神经病学与精神病学]