大曲液化酶活力测定方法的对比研究  

Comparison of Determination Methods of the Liquefying Activity of Daqu Enzyme

作　　者：马群 张梦阳 李宜波 刘忠杰 向征 MA Qun;ZHANG Mengyang;LI Yibo;LIU Zhongjie;XIANG Zheng(Baiyunbian Distillery Co. Ltd., Songzi, Hubei 434200, China)

机构地区：[1]湖北白云边酒业股份有限公司

出　　处：《酿酒科技》2019年第10期68-72,共5页Liquor-Making Science & Technology

摘　　要：对两种测定大曲液化酶活力的方法进行了对比分析。比色法测定大曲液化力和分光光度法测定液化酶活力,方法所依据的原理都是碘遇淀粉后反应产生特殊的颜色变化。但是,比色法检测耗时长,容易出现颜色判断误差,精确度不高。而分光光度法测定液化酶活力,速度快,精确度高。通过本文的对比分析,可以为大曲液化酶活力测定方法的选用提供一定的参考。In this study, two methods for the determination of the liquefying activity of Daqu enzyme were compared. Both colorimetric method and spectrophotometric method are based on the special color reaction between iodine and starch. Colorimetric method is time-consuming, the determination accuracy is not high, and color judgment errors occur easily. However, spectrophotometric method is rapid and has high determination accuracy. Accordingly, spectrophotometric method is a better choice for the determination.

关 键 词：大曲 液化酶活力 比色法 分光光度法 精确度 

分 类 号：TQ925.7[轻工技术与工程—发酵工程] TQ920