Linc00460通过下调miR-654-3p在食管癌中发挥促癌作用  

Linc00460 promotes cancer by down-regulating miR-654-3p in esophageal cancer

作　　者：杨振威[1] 赵倩[2] 庄坤[1] 韩坤[1] 和水祥[2] 刘亚萍[2] YANG Zhen-wei;ZHAO Qian;ZHUANG Kun;HAN Kun;HE Shui-xiang;LIU Ya-ping(Department of Gastroenterology,Xi'an Central Hospital,Xi'an 710004;Department of Gastroenterology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安市中心医院消化内科,陕西西安710004 [2]西安交通大学第一附属医院消化内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2019年第6期871-876,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省重点科研计划资助项目(No.2017SF-255)~~

摘　　要：目的探讨linc00460及miR-654-3p在食管癌中的表达水平及与食管癌预后和生物学功能的关系。方法将121例食管癌患者分为linc00460高表达组及linc00460低表达组,使用卡方检验分析两组患者间临床指标的差异,通过log-rank检验及COX回归模型进行生存分析。通过R软件绘制nomogram;使用C-index进行nomogram的内部检验。用CCK-8实验明确linc00460及miR-654-3p对Eca-109增殖的影响。使用双荧光素酶报告基因实验明确linc00460及miR-654-3p的调控关系。结果linc00460在食管癌中高表达,而miR-654-3p则为低表达;卡方检验结果提示二者表达相关。生存分析提示linc00460及miR-654-3p与食管癌预后相关。CCK-8实验提示linc00460可促进Eca-109的增殖。且linc00460通过与miR-654-3p直接结合发挥生物学功能。结论linc00460及miR-654-3p的表达水平与食管癌的预后有关;linc00460通过下调miR-654-3p在食管癌中发挥促癌作用。Objective To investigate the role and expressions of linc00460 and miR-654-3 p in esophageal cancer so as to further investigate their role in the prognosis and biology of esophageal cancer. Methods We divided 121 patients with esophageal cancer into linc00460 high expression and low expression groups. Chi-square test was used for differential expression analysis and log-rank test and COX model were used for survival analysis. R was used for nomogram plots with C-index for internal validation. CCK-8 was used for proliferation tests of linc00460 and miR-654-3 p. Dual luciferase reporter gene assay was used to investigate the association between linc00460 and miR-654-3 p expression levels. Results Linc00460 was highly expressed in esophageal cancer while miR-654-3 p was lowly expressed. Chi-square tests indicated that the expression of linc00460 was related to the expression of miR-654-3 p. Log-rank indicated that the expressions of linc00460 and miR-654-3 p were associated with the prognosis of esophageal cancer patients. CCK-8 indicated that linc00460 promoted the proliferation and it was directly bound to miR-654-3 p to exert its oncogenic function. Conclusion Linc00460 and miR-654-3 p expression profiles were associated with the prognosis of esophageal cancer patients, and linc00460 promoted cancer progression through downregulating miR-654-3 p.

关 键 词：长链非编码RNA linc00460 miR-654-3p 食管癌 增殖 

分 类 号：R735.1[医药卫生—肿瘤]