宫颈病变中C-MYC、hTERC表达及其与SPR技术检测HP V结果的相关性分析  

Expression of c-myc and hTERC in Cervical Lesions and Its Correlation with SPR Detection of HPV Results

作　　者：刘洋[1] 杨德红 孙春意[1] 黄娅娟 杨莹莹[1] 周红林[1] LIU Yang;Yang De-hong;Sun Chun-Yi;HUANG Ya-juan;YANG Ying-ying;ZHOU Hong-lin(Dept.of Gynecology,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China)

机构地区：[1]昆明医科大学第二附属医院妇科

出　　处：《昆明医科大学学报》2019年第10期85-88,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目（81860515）;云南省卫计委人才培养资助项目（H-201629）;云南省科技厅-昆明医科大学联合专项基金资助项目[2018FE001（-055）];云南省卫生科技计划项目（2017NS277,2016NS287,2016NS286,2014NS092,2014NS091）

摘　　要：目的 探讨C-MYC基因、hTERC基因在宫颈病变中与HPV病毒感染的关联性。方法 宫颈癌筛查患者均行HC2-HPV及C-MYC基因检测,任何具有阳性结果的患者均通过病理组织学检查作为诊断金标准。结果 在正常对照组、CINⅠ、CINⅡ/CINⅢ、ICC组中,C-MYC基因的显现率各组间比较,差异有统计学意义(P<0.05)。随组织病变的严重度的增加,C-MYC基因呈阳性扩增和SPR-HPV检出阳性率均有不同水平的上升,且二者呈正相关。结论 hTERC基因、C-MYC基因的不正常扩增和HPV感染与宫颈恶性病的发生密切相关。hTERC和c-MYC的不正常扩增率随组织病变的严重度增加而增加。两者之间互补,适用于宫颈病变的早起筛查和高级别病变风险的预估。Objective To study the correlation between c-MYC gene and hTERC gene and HPV infection in cervical lesions. Methods Patients with cervical cancer screening were performed for detection of SPR-HPV and C-MYC gene. Any positive results should be carried out cervical biopsy.Pathological results were the gold standard for diagnosis in packet.Result The differences of the expression of C-MYC gene and hTERC gene were statistically significant(P < 0.05). The C-MYC gene and hTERC gene had amplified and the positive rate of SPR-HPV had increased, when the pathological tissue lesions aggravated. Both were positively correlated.Conclusion The abnormal amplification of C-MYC gene and hTERC gene is closely related to HPV infection in the development of cervical cancer. hTERC gene and c-MYC gene detected by FISH can be applied in screening of cervical cancer and assessment of the risks of progressing to high-grade lesions.

关 键 词：宫颈病变 人乳头瘤病毒 人髓细胞增生原癌基因 染色体端粒酶基因 表面等离子谐振法 

分 类 号：R737.33[医药卫生—肿瘤]