过氧化氢导致肠上皮细胞线粒体编码三磷酸腺苷合成酶基因损伤的研究  

Mitochondrial encoding ATPase genome damage in intestinal epithelial cells induced by hydrogen peroxide

作　　者：蔡黔[1] 周红[2] 肖光夏[2] 

机构地区：[1]兰州军区兰州总医院烧伤整形科,730050 [2]第三军医大学附属西南医院烧伤研究所

出　　处：《中华创伤杂志》2002年第11期688-691,共4页Chinese Journal of Trauma

基　　金：国家重点基础研究发展规划专项经费资助项目(G19990 5 42 0 2 )

摘　　要：目的　探讨过氧化氢 (H2 O2 )对肠上皮细胞线粒体DNA(mtDNA)编码的三磷酸腺苷(ATP)合成酶基因的损伤作用。　方法　肠上皮细胞株 (SW - 480 )采用 4mmol/LH2 O2 处理后进行mtDNA的提取 ,对ATP合成酶的ATPase6亚基、ATPase8亚基基因进行聚合酶链反应 (PCR)扩增 ,并对PCR产物直接进行测序 ;同时于该时相点测定酶活性。　结果　ATPase8亚基基因在 83 85～85 43段出现大范围的点突变 ;H2 O2 可导致mtDNA突变基因所编码的ATP合成酶活性明显下降。结论　H2 O2 可明显损伤mtDNA编码的ATP合成酶基因 。Objective To investigate the injury effects of hydrogen peroxide on construction and function of mitochondrial DNA (mtDNA) encoding ATPase gene in intestinal epithelial cells. Methods Intestinal epithelial cells SW-480 were treated with saline solution (control) or hydrogen peroxide (H 2O 2), a representative ROS. mtDNA was extracted from the intestinal epithelial cells treated with 4 mmol/L H 2O 2. Polymerase chain reaction (PCR) with different primers of ATPase subunits 6 and 8 (ATPase 6 and ATPase 8) was performed. The products of PCR were directly sequenced. The activities of the enzyme were determined at the same time. Results There were large-scale point mutations from 8385 to 8543 of ATPase 8. H 2O 2 could result in mutagenesis (point and deletion mutations) in mtDNA encoding ATPase 8 and significantly decrease the activity of ATPase. Conclusions H 2O 2 can significantly damage the genome of ATPase encoded by mtDNA, which may result in activity decrease of ATPase

关 键 词：过氧化氢 肠上皮细胞 线粒体编码 ATP合成酶 氧化损伤 自由基 

分 类 号：R363[医药卫生—病理学]