海嘧啶注射剂对SGC-7901人胃癌细胞凋亡的实验研究  

Study of Sea Pyrimidine on apoptosis of human gastric cancer cell SGC-7901

作　　者：季宇彬[1] 高世勇[1] 孔琪[1] 张秀娟[1] 杨宝峰[2] 

机构地区：[1]哈尔滨商业大学,黑龙江哈尔滨150076 [2]哈尔滨医科大学黑龙江哈尔滨,150086

出　　处：《中国海洋药物》2002年第5期13-17,共5页Chinese Journal of Marine Drugs

基　　金：国家科委生命中心博士基金资助(项目编号:96-901-06-10);中国优秀博士后基金资助中博号[1999]17号;黑龙江省杰出青年基金资助J9906;黑龙江省自然科学基金项目资助(项目编号:D9801);国家自然科学基金项目:C03050205;黑龙江省高等学校骨干教师创新能力资助计

摘　　要：目的:揭示海嘧啶的抗肿瘤作用机理。方法:采用流式细胞仪测定海嘧啶对人胃癌细胞凋亡及细胞周期的影响;采用激光扫描共聚焦技术观测海嘧啶对人胃癌细胞内[Ca2+]i的影响及[Ca2+]i变化时Ca2+的来源。结果:海嘧啶可诱导肿瘤细胞凋亡,升高肿瘤细胞内[Ca2+]i浓度,[Ca2+]i升高来源于细胞外钙内流和细胞内钙释放。结论:海嘧啶的抗肿瘤作用机理为诱导肿瘤细胞凋亡,通过开放细胞膜钙通道和引起细胞内钙释放两条途径升高肿瘤细胞内[Ca2+]i,启动细胞凋亡机制,从而诱导肿瘤细胞凋亡。Objective To uncover the anti-tumor mechanism of Sea Pyrimidine(SP), a compound Chinese-Western medicinal preparation containing 5-fluorouracil, extracts of Astragalus membranaceus(Fisch. ) Bunge, Sophora flavescens Ait. And certain sea weed. Methods Effect of SP on SGC-7901 gastric cancer cell cycle and apoptosis were determined by FCM and the source of Ca2+ during intracellular [Ca2+]; change, observed by laser scanning confo-cal microscopy. Results Sea Pyrimidine can induce apoptosis of SGC-7901 tumor cell and elevate its [Ca2+]i level. The source of Ca2+ was originated from extra-cellular [Ca2+]i and intracellular Ca2+ release. Conclusion The anti-tumor mechanism of SP is its action to induce apoptosis of tumor cell through opening of calcium channel and release of intracellular calcium to initiate cellular degradation resulting in tumor cell apoptosis.

关 键 词：实验研究 海嘧啶 人胃癌细胞 细胞凋亡 CA^2+ 

分 类 号：R735.2[医药卫生—肿瘤]